SAB4200317

Anti-OS9 antibody, Mouse monoclonal

clone OS9-8, purified from hybridoma cell culture

• Synonym(s): Anti-ERLEC2, Anti-Endoplasmic reticulum lectin 2, Anti-Endoplasmic reticulum lectin, ERLEC2, Anti-Erlectin 2, Anti-OS-9, amplified in osteosarcoma 9, Anti-Osteosarcoma amplified 9
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: OS9-8, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~97/88 kDa
• species reactivity: human, rat, mouse
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): suitable; western blot: 2-4 μg/mL using HEK-293T cells overexpressing OS9.
• isotype: IgG2b
• UniProt accession no.: Q13438
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... OS9(10956) ; mouse ... Os9(216440) ; rat ... Os9(362891)

Description

General description

Monoclonal Anti-OS9 (mouse IgG2b isotype) is derived from the hybridoma OS9-8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Amplified in osteosarcoma 9 (OS-9) is a N-glycosylated protein, mapped to human chromosome 12q13.3-q14.1. It is ubiquitously expressed in two isoforms OS-9.1 and OS-9.2.

Specificity

Monoclonal Anti-OS9 recognizes human, rat and mouse OS9 isoforms 1 and 2.

Immunogen

synthetic peptide corresponding to the C-terminal region of human OS9, conjugated to KLH. The corresponding sequence is identical in mouse and rat OS9. The antibody recognizes OS9 isoforms 1 and 2.

Application

Monoclonal Anti-OS9 antibody produced in mouse may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Amplified in osteosarcoma 9 (OS-9) is an endoplasmic reticulum (ER)-resident lectin involved in ER quality control. OS9 is upregulated in response to ER stress through activation of the inositol-requiring enzyme 1/X-box binding protein 1 (Ire1/Xbp1) pathway. OS9 associates both with components of the Endoplasmic-reticulum-associated protein degradation (ERAD) machinery and ERAD substrates. It binds to misfolding proteins preventing their secretion from the ER and directing them to the suppressor/enhancer of Lin-12-like (SEL1L)/HMG-CoA reductase degradation protein 1 (HRD1) dislocation and ubiquitylation complex in the ER membrane. Two OS9 spliced variants are amplified in tumors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable