SAB4200315
Anti-EAPP (E2F-Associated Phosphoprotein) antibody, Mouse monoclonal
clone 1E4, purified from hybridoma cell culture
Synonym(s):
Anti-BM036
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
1E4, monoclonal
form
buffered aqueous solution
mol wt
antigen ~42 kDa
species reactivity
human, mouse
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: suitable
western blot: 2.5-5.0 μg/mL using whole extracts of human HEK-293T cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EAPP(55837)
General description
Monoclonal Anti-EAPP (E2F-associated phosphoprotein) (mouse IgG1 isotype) is derived from the hybridoma 1E4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein. The EEAP gene is mapped to human chromosome 14q13.1. The encoded protein localizes to the nucleus and is present throughout the cell cycle except during mitosis.
Specificity
Monoclonal Anti-EAPP (E2F-associated phosphoprotein) recognizes human and mouse EAPP.
Immunogen
fusion protein corresponding to the N-terminus of human EAPP
Application
Anti-EAPP (E2F-Associated Phosphoprotein) antibody, Mouse monoclonal is suitable for immunoblotting and immunofluorescence.
Biochem/physiol Actions
E2F-associated phosphoprotein (EAPP) interacts with several members of the E2F family of proteins. The E2F family plays a crucial role in the control of the cell cycle and in the action of tumor suppressor proteins. It functions to modulate E2F-regulated transcription and stimulates proliferation. Increased levels of EEAP is observed in most transformed human cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Yang Xue et al.
Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 43(7), 1248-1255 (2015-07-01)
To report on the molecular genetic analysis of a Chinese patient with Maffucci syndrome. Using the genomic DNA extracted from the patient's hemangioma sample, the coding exons and exon/intron splice junctions of the IDH1 and IDH2 genes were amplified by
Michael Novy et al.
Molecular biology of the cell, 16(5), 2181-2190 (2005-02-18)
E2F transcription factors play an essential role in cell proliferation and apoptosis and their activity is frequently deregulated in human cancers. In a yeast two-hybrid screen we identified a novel E2F-binding protein. Due to its strong phosphorylation we named it
Ludwig Schwarzmayr et al.
The international journal of biochemistry & cell biology, 40(12), 2845-2853 (2008-07-01)
The E2F-associated phosphoprotein (EAPP) is a ubiquitous nuclear protein that interacts with the activating members of the E2F family of transcription factors and increases the activity of several cell-cycle regulated promoters in an E2F-dependent manner. Our previous studies also showed
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