The proline-rich protein 5 (PRR5) gene is mapped to human chromosome 22q13.31. PRR5, also known as Protor-1, is a conserved proline-rich protein highly expressed in kidney. PRR5 is a component of the mammalian target of rapamycin complex 2 (mTORC2). Alternative splicing of the gene gives rise to five transcripts encoding different isoforms.
Specificity
Anti-PRR5 recognizes human PRR5.
Immunogen
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Application
Anti-PRR5 (C-terminal) antibody produced in rabbit is suitable for immunoblotting.
Biochem/physiol Actions
The proline-rich protein 5 (PRR5) gene acts as a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. It regulates platelet-derived growth factor (PDGF) receptor β expression and PDGF signaling to protein kinase B (Akt) and S6 Kinase 1 (S6K1).
Physical form
synthetic peptide corresponding to the C-terminal region of human PRR5, conjugated to KLH. The corresponding sequence differs by a single amino acid in mouse and by 2 amino acids in rat.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended.If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Loss of heterozygosity on chromosome 22q13.31 is a frequent event during human breast and colorectal carcinogenesis. Herein we characterize a novel gene at chromosome 22q13.31 designated PRR5. Alternative promoter usage and splicing converge to generate five PRR5 transcript variants with
The Journal of biological chemistry, 282(35), 25604-25612 (2007-06-30)
The protein kinase mammalian target of rapamycin (mTOR) plays an important role in the coordinate regulation of cellular responses to nutritional and growth factor conditions. mTOR achieves these roles through interacting with raptor and rictor to form two distinct protein
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