SAB4200309
Anti-Recoverin (C-terminal) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody
Synonym(s):
Anti-RCVRN, Anti-CAR (cancer-associated retinopathy), Anti-RXV1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.44
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biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~23 kDa
species reactivity
human, rat
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.5 mg/mL
technique(s)
western blot: 1.5-3.0 μg/mL using rat brain extracts (S1 fraction) and SH-SY5Y cell extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RCVRN(5957)
mouse ... Rcvrn(19674)
rat ... Rcvrn(140936)
General description
The recoverin (RCVRN) gene is mapped to human chromosome 17p13.1. Recoverin is a neuronal calcium (Ca2+) sensor (NCS) protein and contains four EF-hand Ca2+-binding sites. Recoverin is mainly localized in the retina, in cone and rod photoreceptors, cone bipolar cells, and in a subpopulation of cells in the ganglion cell layer.
Specificity
Anti-Recoverin (C-terminal) specifically recognizes human and rat recoverin.
Immunogen
synthetic peptide corresponding to a sequence at the C-terminus of rat recoverin, conjugated to KLH. The corresponding sequence is identical in mouse recoverin and highly conserved (86% sequence identity) in human recoverin.
Application
Anti-Recoverin (C-terminal) antibody produced in rabbit is suitable for immunoblotting.
Biochem/physiol Actions
Recoverin plays an important role in phototransduction. The neuronal calcium (Ca2+) sensor (NCS) proteins are involved in a variety of Ca2+-dependent signal transduction processes in neurons. Recoverin is expressed in early stages of development, thus providing an effective marker to assess the development of photoreceptors in the retina. Recoverin serves as a Ca2+ -dependent regulator of rhodopsin kinase (GRK1)/rhodopsin kinase that catalyzes the phosphorylation and desensitization of visual receptor rhodopsin. Recoverin has been suggested to interact with additional NCS proteins, including caldendrin, to modulate its activity. Recoverin has also been identified as an autoantigen in the degenerative disease of the retina cancer-associated neuropathy (CAR). The aberrant expression of recoverin in malignant tumors has been shown to cause an autoimmune response in some cancer patients that leads to the development of paraneoplastic CAR syndrome.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Ivan I Senin et al.
Journal of molecular biology, 330(2), 409-418 (2003-06-26)
Recoverin is a neuronal calcium sensor protein that plays a crucial role in vertebrate phototransduction. It undergoes a Ca(2+)-myristoyl switch when Ca(2+) binds to its two functional EF-hand motifs (EF-hands 2 and 3), each present in one of recoverin's two
Hiroshi Ohguro et al.
American journal of ophthalmology, 137(6), 1117-1119 (2004-06-09)
To report clinical and immunologic aspects of cancer-associated retinopathy (CAR). Observational consecutive case series. A retrospective review was made of 18 consecutive patients with cancer-associated retinopathy who had antiretinal antibody determination by Western blot testing. Clinically, a variety of ophthalmic
Ramona Fries et al.
Journal of neurochemistry, 113(5), 1150-1162 (2010-03-20)
Caldendrin and recoverin are Ca(2+)-sensor proteins operating in neuronal systems. In a search for novel binding partners of recoverin, we employed an affinity column and identified caldendrin as a possible interaction partner. Caldendrin and recoverin co-localized in the retina in
Emine Günhan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(4), 1383-1389 (2003-02-25)
An antibody against recoverin, the calcium-binding protein, labels photoreceptors, cone bipolar cells, and a subpopulation of cells in the ganglion cell layer. In the present study, we sought to establish the origin and identity of the cells expressing recoverin in
Konstantin E Komolov et al.
Journal of neurochemistry, 110(1), 72-79 (2009-05-22)
Recoverin is suggested to inhibit rhodopsin kinase (GRK1) at high [Ca(2+)] in the dark state of the photoreceptor cell. Decreasing [Ca(2+)] terminates inhibition and facilitates phosphorylation of illuminated rhodopsin (Rh*). When recoverin formed a complex with GRK1, it did not
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