SAB4200306

Anti-mLST8/GBL (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-G protein beta subunit-like, Anti-GBL, Anti-GbetaL, Anti-LST8, Anti-LST8 homolog (S. cerevisiae), Anti-MGC111011, Anti-MLST8, Anti-MTOR associated protein, Anti-POP3, Anti-TORC subunit LST8, Anti-WAT1, Anti-gable, Anti-mammalian lethal with SEC13 protein 8, Anti-protein GbetaL, Anti-target of rapamycin complex subunit LST8
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous suspension
• mol wt: antigen ~38 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 10-20 μg using lysates of human A431 cells; western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over expressing human mLST8
• UniProt accession no.: Q9BVC4
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MLST8(64223) ; mouse ... Mlst8(56716) ; rat ... Mlst8(64226)

Description

General description

The mammalian lethal with SEC13 protein 8 (MLST8) gene is mapped to human chromosome 16p13.3. mLST8 also known as GβL (G protein β-subunit-like protein) consists of seven WD40 (tryptophan-aspartic acid/ β-transducin) repeats and has similar sequence homology with the β subunits of heterotrimeric G proteins. mLST8 is a subunit of the mammalian target of rapamycin (mTOR) signaling complexes, mTORC1 and mTORC2. mLST8 is conserved among all eukaryotes and is a widely expressed protein.

Specificity

Anti-mLST8/GBL (C-terminal) recognizes human mLST8.

Immunogen

synthetic peptide corresponding to a sequence located near the C-terminus of human mLST8, conjugated to KLH. The corresponding sequence is identical in mouse, rat, canine, bovine and monkey

Application

Anti-mLST8/GBL (C-terminal) antibody produced in rabbit is suitable for immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Mammalian lethal with SEC13 protein 8 (mLST8) stimulates the mammalian target of rapamycin (mTOR) kinase function by associating with the kinase domain of mTOR. mLST8 is shown to be a critical molecule in the nutrient-sensitive raptor-mTOR interaction. This protein might also be involved in the raptor-mediated inhibition of mTOR kinase activity. mLST8 is known to mediate the phosphorylation of S6 Kinase 1 (S6K1), in vivo. This infers the role of mLST8 in positively regulating the mTOR pathway. Overexpression of the mLST8 gene has been observed in hepatocellular carcinoma (HCC).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable