SAB4200302

Anti-YY1 antibody produced in rabbit

IgG fraction of antiserum

• Synonym(s): Anti-Delta transcription factor, Anti-INO80S, Anti-NF-E1, Anti-Transcriptional repressor protein YY1, Anti-Yin and Yang 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~55 kDa
• species reactivity: rat, human
• technique(s): western blot: 1:1000-1:2000 using lysates of HEK-293T cells over expressing human YY1.
• UniProt accession no.: P25490
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... YY1(7528) ; rat ... Yy1(24919)

Description

General description

The Yin Yang-1 (YY1) gene encodes a ubiquitous, conserved, multifunctional zinc-finger transcription factor. It is a phosphoprotein and a member of the polycomb group protein family. The YY1 gene is mapped to human chromosome 14q32.2. Alternative splicing of the gene produces eight different transcripts. The YY1 protein possesses four C2H2-type zinc-finger motifs and two specific domains as an activator or a repressor.

Specificity

Anti-YY1 recognizes human YY1.

Immunogen

synthetic peptide corresponding to an Internal region of human YY1, conjugated to KLH. The corresponding sequence is identical in rat.

Application

Anti-YY1 antibody produced in rabbit is has been used in immunofluorescence[1]. It is suitable for immunoblotting.

Biochem/physiol Actions

Yin Yang-1 gene (YY1) significantly participates in hematopoiesis and cell cycle control. The YY1 protein regulates initiation, activation, or repression of transcription of a variety of genes required for cell growth, development, differentiation, or tumor suppression, depending upon the context in which it binds. YY1 overexpression is associated with cellular proliferation, resistance to Fas ligand-mediated apoptosis, tumorigenesis and metastatic prostate cancer.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable