SAB4200301

Monoclonal Anti-Leupaxin antibody produced in mouse

clone Leu133, tissue culture supernatant

• Synonym(s): Anti-LDPL, Anti-LPXN
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: tissue culture supernatant
• antibody product type: primary antibodies
• clone: Leu133, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~43 kDa
• species reactivity: monkey, human, mouse
• technique(s): immunocytochemistry: suitable; western blot: 1:8000-1:16,000 using human Ramos cell extracts
• isotype: IgM
• UniProt accession no.: O60711
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LPXN(9404) ; mouse ... Lpxn(107321)

Description

General description

Monoclonal Anti-Leupaxin (mouse IgM isotype) is derived from the hybridoma Leu133 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to a sequence at the N-terminus human Leupaxin conjugated to KLH. Leupaxin is a LIM (Lin11, Isl-1 and Mec-3) domain phosphotyrosine protein preferentially expressed in lymphoid tissues and hematopoietic cells. Leupaxin shares homology with the focal adhesion protein paxillin, in the LIM and LD (leucine/aspartate repeat) protein interaction domains. Leupaxin is expressed in cell types that lack FAK, such as human macrophages.

Immunogen

peptide corresponding to a sequence at the N-terminus human Leupaxin, conjugated to KLH. The corresponding sequence is identical in human leupaxin 1 and 2 isoforms.

Application

Monoclonal Anti-Leupaxin antibody produced in mouse has been used in immunoblotting and immunocytochemistry.

Biochem/physiol Actions

Leupaxin LIM domains have been shown to mediate localization to focal contacts and LD motifs have been implicated in focal adhesion kinase (FAK) and vinculin binding, resulting in the localization of FAK to focal adhesions. Leupaxin and protein tyrosine kinase 2 β (PYK2) are both preferentially expressed in leukocytes where they regulate cell type-specific signaling complex. Leupaxin can form a complex with PYK2 and PTP-PEST in several cell types including monocytes and osteoclasts and in prostate cancer cells. In osteoclasts, leupaxin has been suggested to be a critical nucleating component of the osteoclast podosomal signaling complex in the adhesion zone. Leupaxin is expressed in prostate cancer and is thought to play a role in adhesion and invasion of prostate carcinoma cells.

Physical form

Solution containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable