SAB4200300
Anti-DKK1 antibody, Mouse monoclonal
clone DKK1, purified from hybridoma cell culture
Synonym(s):
ANTI-SK, Anti-DICKKOPF homolog 1
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
DKK1, monoclonal
form
buffered aqueous solution
mol wt
antigen apparent mol wt 29/36 kDa (doublet)
antigen predicted mol wt ~29 kDa
species reactivity
mouse, human
concentration
~1.0 mg/mL
technique(s)
western blot: 1.0-2.0 μg/mL using human A549 serum free condition medium.
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DKK1(22943)
General description
Monoclonal Anti-DKK1 (mouse IgG2a isotype) is derived from the hybridoma DKK-33 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide. The Dickkopf (DKK) family comprises five conserved members DKK1-4 and a DKK3-related member Dickkopf-like protein 1 (DKKL1/ Soggy). DKK1-4 encodes for secreted glycoproteins. DKK1 protein contains an N-linked glycosyl group on its carboxy terminal region. The DKK1 gene is mapped to human chromosome 10q21.1.
Specificity
Monoclonal Anti-DKK1 recognizes human and mouse DKK1.
Immunogen
peptide corresponding to a sequence at the N-terminus human DKK1, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse DKK1.
Application
Anti-DKK1 antibody, Mouse monoclonal is suitable for immunoblotting.
Biochem/physiol Actions
Dickkopf1 (DKK1) is a secreted inhibitor of the canonical wnt (wingless-related integration site) signaling pathway that modulates this pathway during embryonic development, including head induction, skeletal development, and limb patterning. DKK1, -2, and -4 have been shown to inhibit wnt signaling by interacting with and antagonizing low-density lipoprotein receptor-related protein (LRP) 5 and LRP6. In addition to LRP5/6, DKK1 binds to kringle containing transmembrane protein (Kremen1 and Kremen2) receptors. The ternary DKK/LRP/Krm complex is rapidly endocytosed, resulting in the removal of the LRP5/6 receptors from the cell membrane and in prolonged inhibition of wnt signaling. DKK1 is also implicated in several diseases including osteoporosis, arthritis, and cancer and is a potential therapeutic target for the treatment of these diseases.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8°C for up to onemonth. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Mitchell E Menezes et al.
International journal of cancer, 130(7), 1477-1483 (2011-09-29)
Dickkopf1 (DKK1), a secreted inhibitor of the Wnt/β-catenin pathway, is a negative regulator of bone formation. DKK1 acts as a switch that transitions prostate cancer bone metastases from osteolytic to osteoblastic and also is an active indicator of poor outcome
M Cristina Nostro et al.
Development (Cambridge, England), 138(5), 861-871 (2011-01-29)
The generation of insulin-producing β-cells from human pluripotent stem cells is dependent on efficient endoderm induction and appropriate patterning and specification of this germ layer to a pancreatic fate. In this study, we elucidated the temporal requirements for TGFβ family
José M López et al.
Bone, 43(2), 405-409 (2008-06-10)
Craniotubular hyperostosis are a group of high bone mass disorders related to mutations in the LRP5 and SOST genes, although other causative genes remain to be identified. Little is known about the bone turnover and the response to T3 or
Melchiorre Cervello et al.
Cell cycle (Georgetown, Tex.), 11(15), 2843-2855 (2012-07-18)
Sorafenib, a multikinase inhibitor, recently received FDA approval for the treatment of advanced hepatocellular carcinoma (HCC). However, as the clinical application of sorafenib evolves, there is increasing interest in defining the mechanisms underlying its anti-tumor activity. Considering that this specific
Sigrid Haugen et al.
Frontiers in endocrinology, 9, 236-236 (2018-06-06)
Primary human osteoblasts and osteoclasts incubated in a rotating coculture system without any scaffolding material, form bone-like tissue that may be used to evaluate effects of various compounds on mechanical strength. Circulating adiponectin has been found to be negatively associated
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service