SAB4200299

Anti-Contactin-2 (N-terminal antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-AXT, Anti-Axonin-1, Anti-Cntn2, Anti-Contactin-2(axonal), Anti-TAG-564, Anti-TAG1, Anti-Tax
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~135 kDa
• species reactivity: human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): western blot: 1.5-3.0 μg/mL using HEK-293T cells overexpressing human contactin-2.
• UniProt accession no.: Q02246
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CNTN2(6900)

Description

General description

Anti-Contactin-2 (N-terminal) is produced in rabbit using as immunogen a synthetic peptide conjugated to KLH. Contactin-2 (CNTN2), also known as TAG-1, axonin-1, Tax, astaxanthin (AXT), is an axonal glycoprotein, encoded by the gene mapped to human chromosome 1q32.1. The encoded protein is a member of neuronal cell adhesion molecules of the immunoglobulin superfamily (IgCAMs). The protein contains six extracellular immunoglobulin-like domains and four fibronectin III-like domains.

Contactin-2 is a cell adhesion molecule produced abundantly in Purkinje cells that make up the distal component of cardiac conduction system. It is anchored to the cell membrane by glycan phosphatidyl inositol and is secreted into the extra cellular matrix.

Specificity

Anti-Contactin-2 (N-terminal) specifically recognizes human contactin-2.

Immunogen

Anti-Contactin-2 (N-terminal) antibody produced in rabbit specifically recognizes the N terminal region of human contactin-2.

synthetic peptide corresponding to a sequence at the N-terminal region of human contactin-2, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse contactin-2.

Biochem/physiol Actions

Contactin-2 is a critical for neuronal patterning and ion channel clustering. It has key functions in neurogenesis and morphogenetic functions. Contactin-2 repairs the neurons and its expression increases highly post central nervous system injury.

The interaction of CNTN2 with APP increases γ-secretase-mediated release of APP intracellular fragment (AICD), inducing a Fe65- dependent suppression of neurogenesis. Mutation in the gene causes Alzheimer′s disease (AD).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable