SAB4200295

Anti-Glycogen Synthase 2 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-GYS2, Anti-Glycogen Synthase 2 (liver)
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~85 kDa (rat and mouse); antigen ~95 kDa (human)
• species reactivity: mouse, rat, human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): western blot: 1.5-3 μg/mL using extracts of mouse liver (S1 fraction), rat liver (S1 fraction) and of HEK-293T cells overexpressing human GYS2.
• UniProt accession no.: P54840
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GYS2(2998) ; mouse ... Gys2(232493) ; rat ... Gys2(25623)

Description

General description

The glycogen synthase 2 (GYS2) gene is mapped to human chromosome 12p12.1. GS or GYS exists in two isoforms of GYS-1, found in muscle and GYS-2, which is specific to the liver. The muscle GS form is also expressed in several tissues, including adipose tissue, kidney, spleen and in the nervous system.

Specificity

Anti-Glycogen Synthase 2 (C-terminal), specifically recognizes human, mouse and rat glycogen synthase 2.

Immunogen

synthetic peptide corresponding to a sequence located at the C-terminus of human glycogen synthase 2 (GYS2), conjugated to KLH. The corresponding sequence is highly conserved in rat and mouse GYS2 (84% identity).

Application

Anti-Glycogen Synthase 2 (C-terminal) antibody may be used for immunoblotting at a working concentration of 1.5-3.0 μg/ml using S1 extracts of mouse liver, rat liver and HEK-293T cells over expressing human GYS2.

Biochem/physiol Actions

Glycogen synthase catalyzes the incorporation of α-1,4-linked glucose units into glycogen chains.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable