SAB4200294
Anti-DCLK1 (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
Synonym(s):
Anti-DCAMKL1, Anti-DCDC3A, Anti-DCLK, Anti-Doublecortin and CaM kinase-like 1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.44
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~81 kDa
species reactivity
rat, mouse
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 3-6 μg using extracts of postnatal (PN3) rat brain (S1 fraction).
western blot: 0.05-0.1 μg/mL using extracts of postnatal (PN3) mouse brain (S1 fraction).
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Dclk1(13175)
rat ... Dclk1(83825)
General description
Doublecortin-like kinase 1 (DCLK1) is a microtubule-associated kinase. This Ser/Thr kinase is widely expressed in the post-mitotic neurons in the developing nervous system. DCLK1 is highly expressed during embryogenesis and is localized to neuronal growth cones. DCLK1 consists of an N-terminal doublecortin (DC) domain and a C-terminal Ser/Thr kinase domain that resembles members of the family of Ca2+/calmodulin-dependent protein kinases, but lacks a calmodulin-binding site. The doublecortin (DC) domain is responsible for its localization to microtubules. Four major DCLK1 transcripts have been described in rodents.
Specificity
Anti-DCLK1 (C-terminal) specifically recognizes rat and mouse DCLK1.
Immunogen
synthetic peptide corresponding to a sequence located at the C-terminal of mouse DCLK1, conjugated to KLH. The corresponding sequence is identical in mouse DCLK1 isoform 2 and rat DCLK1.
Application
Anti-DCLK1 (C-terminal) antibody is suitable for use in western blot (0.05-0.1 μg/mL using S1 fraction extracts of postnatal (PN3) mouse brain) and immunoprecipitation (3-6 μg using S1 fraction extracts of postnatal (PN3) rat brain).
Biochem/physiol Actions
DCLK kinase domain is susceptible to cleavage by the Ca2+-dependent protease calpain, suggesting that a Ca2+-responsive mechanism is involved in regulating the DCLK1 kinase activity during embryogenesis. DCLK1 has also been identified as a putative intestinal stem cell marker.
Doublecortin like kinase 1 (DCLK1) is a neuronal transmembrane enzyme and may regulate cortical development, nerve cell migration and microtubule polymerization. This enzyme is similar to doublecortin (DCX), but DCLK1 additionally functions as a Ca2+/calmodulin-dependent protein kinase that can phosphorylate myelin basic protein and itself .
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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H A Burgess et al.
The Journal of biological chemistry, 276(39), 36397-36403 (2001-07-27)
Doublecortin-like kinase (DCLK) is widely expressed in postmitotic neurons throughout the embryonic nervous system. DCLK consists of an N-terminal doublecortin domain, responsible for its localization to microtubules, and a C-terminal serine-threonine kinase domain. Here we report that DCLK is a
H A Burgess et al.
Molecular and cellular neurosciences, 16(5), 529-541 (2000-11-21)
Doublecortin-like kinase (DCLK) shares sequence similarity to Doublecortin (DCX) in its N-terminal region. It contains the evolutionary conserved DC repeat motif as well a C-terminal kinase domain. Ectopic expression of DCLK in COS cells results in colocalization with microtubules, and
Françoise Phan Dinh Tuy et al.
Developmental neuroscience, 30(1-3), 171-186 (2007-12-14)
The doublecortin (DCX) gene, mutated in X-linked human lissencephaly, has 2 close paralogs, doublecortin-like kinase 1 and 2 (Dclk1 and 2). In this study we attempted to better understand the dramatic differences between human and mouse DCX/Dcx-deficient phenotypes, focusing on
Randal May et al.
Stem cells (Dayton, Ohio), 26(3), 630-637 (2007-12-07)
In the gut, tumorigenesis arises from intestinal or colonic crypt stem cells. Currently, no definitive markers exist that reliably identify gut stem cells. Here, we used the putative stem cell marker doublecortin and CaM kinase-like-1 (DCAMKL-1) to examine radiation-induced stem
N Matsumoto et al.
Genomics, 56(2), 179-183 (1999-03-03)
Human DCAMKL1, also known as KIAA0369, is a homologue of DCX (Xq22. 3), a gene associated with X-linked lissencephaly and subcortical band heterotopia. This suggests that DCAMKL1 may play a role in neuronal migration. The gene also shows similarity to
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