SAB4200289

Anti-Dystrobrevin-β (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-DTNB
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~72 kDa
• species reactivity: rat, human
• concentration: ~1.5 mg/mL
• technique(s): immunohistochemistry: 20-40 μg/mL using formalin-fixed, paraffin-embedded rat kidney.; indirect immunofluorescence: 2-4 μg/mL using HEK-293T cells overexpressing human dystrobrevin-β.; western blot: 1.5-3.0 μg/mL using lysates of HEK-293T cells overexpressing human dystrobrevin-β.
• UniProt accession no.: O60941
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DTNB(1838) ; rat ... Dtnb(362715)

Description

General description

The dystrobrevin-β(DTNB) gene is mapped to human chromosome 2p23.3. The encoded protein belongs to the dystrobrevin (DB) subfamily of the dystrophin-related protein family. DB is a component of the dystrophin-glycoprotein complex (DGC) that is located at the muscle sarcolemma and forms a transmembrane link between the cytoskeleton and the basal lamina. β-DB is confined to non-muscle tissues and predominantly expressed in the kidney and brain. β-DB is linked to dystrophin and syntrophin isoforms in the hippocampus, cortex, and Purkinje cells. It is abundant in the post-synaptic densities (PSDs).

Specificity

Anti-Dystrobrevin-b(N-terminal) specifically recognizes human and rat dystrobrevin-b.

Immunogen

synthetic peptide corresponding to a sequence located at the N-terminus of human dystrobrevin-b (DTNB), conjugated to KLH. The corresponding sequence is identical in human DTNB isoforms 1-5, and highly conserved (single amino acid substitution) in rat and mouse DTNB.

Application

Anti-Dystrobrevin-β (N-terminal) antibody produced in rabbit is suitable for:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions

Dystrobrevin-β (β-DB) may be significantly involved in assembling and supporting the dystrophin-associated protein complex (DPC)-like complexes in non-muscle tissues. It is known to interact with the neuronal kinesin heavy chain Kif5A and Kif5B in the brain, suggesting that β-DB might act as a motor protein receptor involved in the transport of dystrophin-glycoprotein complex (DGC) components to specific sites in the cell.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable