SAB4200288

Anti-Dystrobrevin-β antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-DTNB
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~72 kDa
• species reactivity: human, rat
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): immunohistochemistry: 20-40 μg/mL using formalin-fixed, paraffin-embedded rat kidney; immunoprecipitation (IP): 15-30 μg using HEK-293T cells overexpressing human dystrobrevin-β; indirect immunofluorescence: 2-4 μg/mL using HEK-293T cells overexpressing human dystrobrevin-β; western blot: 1.5-3.0 μg/mL using lysates of HEK-293T cells overexpressing human dystrobrevin-β
• UniProt accession no.: O60941
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DTNB(1838) ; rat ... Dtnb(362715)

Description

General description

The Dystrobrevin (DTNB) gene is mapped to human chromosome 2p23.3. β-DB belongs to the dystrobrevin (DB) subfamily of the dystrophin family. β-DB is found only in non-muscle tissues, and is predominantly expressed in the kidney and brain. It is also highly enriched in postsynaptic densities (PSDs). β-DB is a component of the dystrophin-protein complex (DPC).

Specificity

Anti-Dystrobrevin-b specifically recognizes human and rat dystrobrevin-b.

Immunogen

synthetic peptide corresponding to a sequence located near the N-terminus of human dystrobrevin-b (DTNB), conjugated to KLH. The corresponding sequence is identical in human DTNB isoforms 1-5, and highly conserved (single amino acid substitution) in rat and mouse DTNB.

Application

Anti-Dystrobrevin-β antibody produced in rabbit is suitable for:

• immunoblotting
• immunofluorescence
• immunoprecipitation
• immunohistochemistry

Biochem/physiol Actions

β-Dystrobrevin (β-DB) is essential for the maintenance and organization of DPC-like complexes in non-muscle tissues. In the brain, β-DB interacts with dystrophin in the cortex, hippocampus, and Purkinje cells. It forms complexes with Dp71 for membrane localization and utrophin. β-DB is associated with the neuronal kinesin heavy chains, Kif5A and Kif5B, in the brain. This infers the role β-DB as a motor protein receptor involved in the site-specific cellular transportation of dystrophin-associated protein complex (DPC) components.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable