Monoclonal Anti-LYN (mouse IgG2b isotype) is derived from the hybridoma LYN-27 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide. The LYN gene is mapped to human chromosome 8q12.1. Lyn is an important Src-family kinase (SFK) expressed in B lymphocytes. Lyn contains an N-terminal unique domain, SH3, SH2, and tyrosine kinases domain. Lyn exists as two isoforms, p53 and p56, arising from alternate splicing of exon 2.
Specificity
Monoclonal Anti-LYN recognizes human and mouse LYN.
Immunogen
peptide corresponding to a sequence within human LYN, conjugated to KLH. This sequence is identical in human LYN A and B isoforms, and highly conserved in rat and mouse LYN (94%)
Application
Anti-LYN antibody, Mouse monoclonal is suitable for immunoblotting and immunocytochemistry.
Biochem/physiol Actions
The tyrosine-protein kinase Lyn acts as both a positive and negative regulator of B cell receptor signaling pathways. Interestingly, it is a critical mediator in many pathways of B cell activation through the cluster of differentiation 19 (CD19) and CD180. It is shown that Lyn activity is associated with the pathological conditions arising due to the LYN gene related loss of function and gain of function in mice. It might cause lethal antibody-mediated autoimmune diseases and myeloid neoplasia in mice.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Lyn, one of several Src-family tyrosine kinases in immune cells, is noted for its ability to negatively regulate signaling pathways through phosphorylation of inhibitory receptors, enzymes, and adaptors. Somewhat paradoxically, it is also a key mediator in several pathways of
In immune cells, Src-family kinases have been implicated as critical regulators of a large number of intracellular signaling pathways. Studies of gene knockout mice lacking various family members have both confirmed the requirement for these kinases in some pathways and
In a newly diagnosed patient with acute myeloid leukemia (AML) and complex cytogenetics and negative for gene mutations associated with myeloid neoplasms, RNA sequencing by next-generation sequencing (NGS) through a large cancer-related gene panel showed ETV6-LYN leukemic fusion transcript. Breakpoint
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