SAB4200284
Anti-Neurocalcin-δ antibody produced in rabbit
~0.5 mg/mL, affinity isolated antibody
Synonym(s):
Anti-NCALD
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.44
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~22 kDa
species reactivity
human, mouse, rat
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~0.5 mg/mL
technique(s)
western blot: 0.5-1.0 μg/mL using mouse brain extract (S1 fraction) and lysates of HEK-293T cells over-expressing human neurocalcin-δ.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NCALD(83988)
mouse ... Ncald(52589)
rat ... Ncald(553106)
General description
The neurocalcin-δ (NCALD) gene is mapped to human chromosome 8q22.3. The encoded protein belongs to a large family of neuronal calcium (Ca2+) sensor (NCS) proteins and is a member of visinin-like protein (VILIP) subfamily. Neurocalcin-δ is also one of the EF-hand-containing Ca2+-binding proteins. It is predominantly expressed in the Purkinje cells of the cerebellum and is also present in the olfactory epithelium.
Specificity
Anti-Neurocalcin-d specifically recognizes human and mouse neurocalcin-d.
Immunogen
synthetic peptide corresponding to a sequence located near the N-terminus of human neurocalcin-δ, conjugated to KLH. The corresponding sequence is identical in mouse and rat neurocalcin-δ.
Application
Anti-Neurocalcin-δ antibody produced in rabbit is suitable for immunoblotting.
Biochem/physiol Actions
Neurocalcin-δ (VILIP-3) is a neuronal calcium (Ca2+) sensor. It binds cooperatively to three Ca2+ ions. Neurocalcin-δ influences cyclic guanosine monophosphate (cGMP) signaling in the retina, by acting as a regulator of the retinal guanylate cyclase (retGC1)/ photoreceptor membrane guanylyl cyclase 1 (ROSGC1). Neurocalcin-δ interacts with retGC1 in a calcium (Ca2+) dependent manner. It colocalizes with retGC1 in neurons in the inner plexiform layer of the retina, in amacrine and ganglion cells where it may influence synaptic signaling processes. Neurocalcin-δ has been shown to interact with actin, clathrin, and α- and β-tubulins, indicating that it may be involved with clathrin-coated vesicle traffic. It interacts and activates the olfactory guanylate cyclase ONE-GC, suggesting that it may function in regulating the odorant signaling pathway.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA as stabilizer and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Chih-Ping Chen et al.
Taiwanese journal of obstetrics & gynecology, 56(6), 843-846 (2017-12-16)
We present prenatal diagnosis of an interstitial 8q22.2-q23.3 deletion associated with bilateral cleft lip and palate and intrauterine growth restriction (IUGR) on fetal ultrasound. A 29-year-old, primigravid woman underwent elective amniocentesis at 17 weeks of gestation because of anxiety. Amniocentesis
The calcium sensor protein visinin-like protein-1 modulates the surface expression and agonist sensitivity of the alpha4beta2 nicotinic acetylcholine receptor
Lin L, et al.
The Journal of biological chemistry, 41872-41878 null
Teresa Duda et al.
FEBS letters, 583(8), 1327-1330 (2009-03-25)
In a subset of olfactory epithelium the odorant receptor guanylate cyclase, ONE-GC, is a central transduction component of the cyclic GMP signaling pathway. The odorant binds to the extracellular domain and activates its intracellular catalytic domain to generate the odorant
Venkateswar Venkataraman et al.
Biochemistry, 42(19), 5640-5648 (2003-05-14)
Rod outer segment membrane guanylate cyclase (ROS-GC) transduction system is a central component of the Ca(2+)-sensitive phototransduction machinery. The system is composed of two parts: Ca(2+) sensor guanylate cyclase activating protein (GCAP) and ROS-GC. GCAP senses Ca(2+) impulses and inhibits
Lenka Ivings et al.
The Biochemical journal, 363(Pt 3), 599-608 (2002-04-20)
The neuronal calcium sensors are a family of EF-hand-containing Ca(2+)-binding proteins expressed predominantly in retinal photoreceptors and neurons. One of the family members is neurocalcin delta, the function of which is unknown. As an approach to elucidating the protein interactions
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