SAB4200279

Anti-MAN1B/ MAN1A2 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Mannosidase, alpha, class 1A, member 2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~70 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 1-2 μg/mL using HeLa cells; western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells overexpressing human MAN1A2
• UniProt accession no.: O60476
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAN1A2(10905)

Description

General description

The α-1,2-Mannosidase IB (MAN1B) gene is mapped to human chromosome 1p12. The encoded protein is also known as mannosidase α, class 1A, member 2 (MAN1A2) and is a Golgi resident transmembrane protein that belongs to the glycosyl hydrolase family 47. It is highly conserved among the eukaryotes and the murine MAN1B protein is localized to the Golgi apparatus. MAN1B is a type II membrane protein containing 35 amino acids in the cytoplasmic region, a Ca²⁺ binding consensus sequence, and a single N-glycosylation site.

Specificity

Anti-MAN1B/MAN1A2 (C-terminal) recognizes human MAN1B.

Immunogen

synthetic peptide corresponding to a C-terminal sequence of human MAN1A2, conjugated to KLH. The corresponding sequence differs by a single amino acid in rat and by two amino acids in mouse.

Application

Anti-MAN1B/ MAN1A2 (C-terminal) antibody produced in rabbit is suitable for immunoblotting and immunofluorescence.

Biochem/physiol Actions

α-1,2-Mannosidase IB (MAN1B) is known to mediate protein transport to the Golgi apparatus for processing. MAN1B progressively trims the α-1,2-linked mannose residues from Man₉GlcNAc₂, to form the processing intermediate Man₅GlcNAc₂. α-Mannosidases function at different stages of N-glycan maturation in mammalian cells. This gene might serve as a prognostic indicator in B cell lymphoma.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. Forextended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable