SAB4200278
Anti-MAN1B /MAN1A2 antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
Synonym(s):
Anti-alpha, Anti-class 1A, Anti-mannosidase, Anti-member 2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~70 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells over expressing human MAN1A2.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MAN1A2(10905)
mouse ... Man1a2(17156)
rat ... Man1a2(295319)
General description
α-1,2-Mannosidase IB (MAN1B), also known as mannosidase α, class 1A, member 2 (MAN1A2), is a Golgi resident transmembrane protein that belongs to the glycosyl hydrolase family 47. MAN1B is a type II membrane protein containing 35 amino acids in the cytoplasmic region, a Ca2+ binding consensus sequence, and a single N-glycosylation site. MAN1B is highly conserved among the eukaryotes and the murine MAN1B protein is localized to the Golgi apparatus. The MAN1B gene is mapped to human chromosome 1p12.
Specificity
Anti-MAN1B/MAN1A2 recognizeshuman MAN1B/MAN1A2.
Immunogen
synthetic peptide corresponding to an internal sequence within human MAN1A2, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine and canine.
Application
Anti-MAN1B /MAN1A2 antibody produced in rabbit is suitable for immunoblotting.
Biochem/physiol Actions
α-Mannosidases function at different stages of N-glycan maturation in mammalian cells. α-1,2-Mannosidase IB (MAN1B) progressively trims the α-1,2-linked mannose residues from Man9GlcNAc2, to form the processing intermediate Man5GlcNAc2. MAN1B is responsible for protein transport to the Golgi apparatus for processing. This gene might serve as a prognostic indicator in B cell lymphoma.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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B Becker et al.
European journal of cell biology, 79(12), 986-992 (2001-01-11)
Murine alpha1,2-mannosidase IB is a type II transmembrane protein localized to the Golgi apparatus where it is involved in the biogenesis of complex and hybrid N-glycans. This enzyme consists of a cytoplasmic tail, a transmembrane domain followed by a "stem"
Steven W Mast et al.
Methods in enzymology, 415, 31-46 (2006-11-23)
Alpha-mannosidases in eukaryotic cells are involved in both glycan biosynthetic reactions and glycan catabolism. Two broad families of enzymes have been identified that cleave terminal mannose linkages from Asn-linked oligosaccharides (Moremen, 2000), including the Class 1 mannosidases (CAZy GH family
Plasma cell neoplasms: genetics, pathobiology, and new therapeutic strategies.
Dong Soon Lee et al.
BioMed research international, 2014, 941820-941820 (2015-01-23)
A Herscovics et al.
The Journal of biological chemistry, 269(13), 9864-9871 (1994-04-01)
The amino acid sequence of the specific alpha-mannosidase involved in N-oligosaccharide processing in Saccharomyces cerevisiae was found to have a high degree of similarity to the deduced amino acid sequence of a rabbit liver alpha-mannosidase partial cDNA, demonstrating that processing
Hsiao-Chen Tu et al.
Hepatology communications, 1(3), 230-247 (2018-02-07)
α-1,2 mannosidases, key enzymes in N-glycosylation, are required for the formation of mature glycoproteins in eukaryotes. Aberrant regulation of α-1,2 mannosidases can result in cancer, although the underlying mechanisms are unclear. Here, we report the distinct roles of α-1,2 mannosidase
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