SAB4200271

Anti-EDEM1(internal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-ER degradation enhancer, Anti-mannosidase alpha-like 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 75/65 kDa
• species reactivity: human, mouse
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 2.5-5.0 μg using lysates of HEK-293T cells over expressing mouse EDEM1.; western blot: 2-4 μg/mL using whole extracts of human Jurkat cells.
• UniProt accession no.: Q92611
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EDEM1(9695) ; mouse ... Edem1(192193)

Description

General description

ER degradation-enhancing α-mannosidase like protein 1 (EDEM1) is a putative mannose-binding lectin. EDEM1 is localized to the endoplasmic reticulum (ER) mainly as a soluble glycoprotein.

Immunogen

synthetic peptide corresponding to an internal region within human EDEM1, conjugated to KLH. The corresponding sequence differs by a single amino acid in mouse and by 2 amino acids in rat.

Application

Anti-EDEM1(internal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Over-expression of ER degradation-enhancing α-mannosidase like protein 1 (EDEM1) leads to endoplasmic reticulum (ER)-associated degradation (ERAD) by stimulating the release of terminally misfolded glycoproteins from calnexin. EDEM degrades misfolded glycoproteins in an N-glycan dependent manner. Three EDEM homologues, EDEM1, EDEM2 and EDEM3 are transcriptionally upregulated by activated inositol-requiring enzyme 1 (IRE1)/X-box binding protein 1 (XBP1) branch. It interacts with the COOH-terminus of calnexin and lacks mannosidase activity.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable