recombinant expression Learn more about Antibody Enhanced Validation
concentration
~1.5 mg/mL
technique(s)
indirect immunofluorescence: 1-2 μg/mL using NIH3T3 cells over expressing human tensin2 western blot: 1.5-3.0 μg/mL using lysates of HEK-293T cells over expressing human tensin2
Tensin 2 belongs to the tensin family. Tensins are multidomain proteins that are characterized with homologous C1, protein phosphatases (PTPases), C2, Src homology 2 (SH2) domains and pTyr-binding (PTB) domains. Tensin 2 mRNA is highly expressed in heart, kidney, skeletal muscle and liver.
Immunogen
synthetic peptide located near the N-terminus human tensin 2, conjugated to KLH. The corresponding sequence is identical in human tensin 2 isoforms 2 and 3, and in rat and mouse tensin 2.
Application
Anti-Tensin 2 (N-terminal) antibody produced in rabbit has been used in various immunochemical techniques including immunoblotting and immunofluorescence.
Biochem/physiol Actions
Tensins are a family of focal adhesion proteins that link between the extracellular matrix (ECM) and the cytoskeleton via integrins, and therefore they might play a role in regulating cell shape and motility. Tensin 2 has been shown to be largely absent in a panel of human cancer cell lines and is down-regulated in human kidney cancer. Tensin 2 variant 3 also contributes to the aggressive tumor behavior in human hepatocellular carcinoma (HCC). The protein through its binding domains interacts with several binding partners and with the tumor suppressor gene deleted in liver cancer 1 (DLC1) to regulate DLC1 tumor suppressor activity. In addition, tensin 2 also stops Akt signaling and cell survival. It reduces intracellular PtdIns-3-4-5-P3 levels at the plasma membrane, indicating that in addition to regulating cytoskeletal dynamics, it influences phosphoinositide-Akt signaling via protein phosphatases (PTPases)domain.
Physical form
solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
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