SAB4200263

Anti-PLEKHA7 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Pleckstrin homology domain-containing family A member 7
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~130 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 2.5-5 μg using lysates of HEK- 293T cells overexpressing human PLEKHA7; indirect immunofluorescence: 1.0-2.0 μg/mL using Caco2 cells; western blot: 0.5-1 μg/mL using lysates of HEK- 293T cells overexpressing human PLEKHA7
• UniProt accession no.: Q6IQ23
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PLEKHA7(144100)

Description

General description

The Pleckstrin homology domain-containing family A, member 7 (PLEKHA7) gene is located on the human chromosome 11p15.2-p15.1. The encoded protein is compsed of WW, pleckstrin homology (PH) and coiled-coil (CC) domains. It is a part of cadherin-based protein complex in mammalian epithelial cells.

Specificity

Anti-PLEKHA7 (N-terminal) recognizes human PLEKHA7.

Immunogen

synthetic peptide corresponding to the N- terminal region of human PLEKHA7, conjugated to KLH. The corresponding sequence differs by two amino acids in mouse.

Application

Anti-PLEKHA7 (N-terminal) antibody produced in rabbit is suitable for:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Pleckstrin homology domain-containing family A, member 7 (PLEKHA7) protein is involved in the cadherin-based protein complex mediated anchoring of microtubule minus ends to the zonula adherens (ZA). This process is neccessary for the biogenesis of specialized junction. PLEKHA7 forms complexes with E-cadherin and cadherin associated protein p120-catenin complexes. It significantly aids in the translocation of NEZHA to the ZA.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable