immunoprecipitation (IP): 2.5-5.0 μg using lysates of HEK-293T cells overexpressing human PLEKHA7 western blot: 0.5-1.0 μg/mL using lysates of HEK- 293T cells overexpressing human PLEKHA7
Pleckstrin homology domain-containing family A, member 7 (PLEKHA7) protein contains WW, pleckstrin homology (PH) and coiled-coil (CC) domains. PLEKHA7 protein is a part of cadherin-based protein complex in mammalian epithelial cells. The PLEKHA7 gene is mapped to human chromosome 11p15.2-p15.1.
Specificity
Anti-PLEKHA7 (C-terminal) recognizes human PLEKHA7.
Immunogen
synthetic peptide corresponding to the C- terminal region of human PLEKHA7 conjugated to KLH. The corresponding sequence differs by a single amino acid in mouse.
Application
Anti-PLEKHA7 (C-terminal) antibody produced in rabbit is suitable for immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Pleckstrin homology domain-containing family A, member 7 (PLEKHA7) protein via the cadherin-based protein complex is involved in anchoring the microtubule minus ends to the zonula adherens (ZA). This is essential for the biogenesis of specialized junction. PLEKHA7 binds directly to E-cadherin and cadherin associated protein p120-catenin complexes and appears to be necessary for recruiting NEZHA to the ZA.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions shouldbe discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Epithelial cells contain noncentrosomal microtubules (MTs), whose minus ends are oriented apically. In contrast with the well-known interactions of the minus ends with the centrosome, little is known about the termination site of the noncentrosomal minus ends. Here we show
The zonula adherens (ZA) is a specialized cadherin-based structure found at the contacts between epithelial cells. Meng et al. (2008) now identify a protein complex containing the microtubule minus-end-binding protein Nezha, which provides a critical link between microtubules and cadherins
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