SAB4200261

Anti-FIP1L1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-hFip1, Anti-FIP1, Anti-RHE, Anti-pre-mRNA 3’end processing factor FIP1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~70 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed NIH-3T3 cells; western blot: 1-2 μg/mL using lysates of NIH-3T3 cells
• UniProt accession no.: Q6UN15
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FIP1L1(81608) ; mouse ... Fip1l1(66899) ; rat ... Fip1l1(289582)

Description

General description

The factor interacting with poly(a) polymerase α (PAPOLA) and cleavage and polyadenylation specificity factor 1 (CPSF1) (FIP1L1) gene is located on human chromosome 4q12. The encoded protein is a subunit of CPSF complex. FIP1L1 is composed of an acidic N-terminus region, a proline-rich domain and a C-terminal region of alternating arginines and aspartates (RD domain).

Specificity

Anti-FIP1L1 (N-terminal) recognizes human FIP1L1.

Immunogen

synthetic peptide corresponding to a sequence at the N- terminal region of human FIP1L1, conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Application

Anti-FIP1L1 (N-terminal) antibody produced in rabbit is suitable for immunoblotting and immunofluorescence.

Biochem/physiol Actions

The factor interacting with poly(a) polymerase α (PAPOLA) and cleavage and polyadenylation specificity factor 1 (CPSF1) (FIP1L1) induces the action of U-rich sequence element mediated poly(A) polymerase (PAP). Alterations in the gene results in a FIP1L1- platelet-derived growth factor receptor A (PDGFRA) fusion gene, which have been observed in conditions such as hypereosinophilic syndrome/chronic eosinophilic leukemia (CEL) and in systemic mast cell disease.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up toone month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable