SAB4200260

Anti-FIP1L1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-FIP1, Anti-RHE, Anti-hFip1, Anti-pre-mRNA 3’end processing factor FIP1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~70 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed NIH-3T3 cells; western blot: 2-4 μg/mL using lysates of NIH-3T3 cells
• UniProt accession no.: Q6UN15
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FIP1L1(81608) ; mouse ... Fip1l1(66899) ; rat ... Fip1l1(289582)

Description

General description

The factor interacting with poly(a) polymerase α (PAPOLA) and cleavage and polyadenylation specificity factor 1 (CPSF1) (FIP1L1) is a subunit of CPSF complex. FIP1L1 contains an acidic N-terminal region, a proline-rich domain and a C-terminal region of alternating arginines and aspartates (RD domain). The FIP1L1 gene is mapped to human chromosome 4q12.

Specificity

Anti-FIP1L1 (C-terminal) recognizes human FIP1L1.

Immunogen

synthetic peptide corresponding to a sequence at the C- terminal region of human FIP1L1 conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Application

Anti-FIP1L1 (C-terminal) antibody produced in rabbit is suitable for immunoblotting and immunofluorescence.

Biochem/physiol Actions

The factor interacting with poly(a) polymerase α (PAPOLA) and cleavage and polyadenylation specificity factor 1 (CPSF1) (FIP1L1) is known to promote the U-rich sequence element mediated poly(A) polymerase (PAP) action. Genetic rearrangements that result in a FIP1L1- platelet-derived growth factor receptor A (PDGFRA) fusion gene have been identified in patients with hypereosinophilic syndrome/chronic eosinophilic leukemia (CEL) and in systemic mast cell disease.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up toone month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable