SAB4200259
Anti-CPSF6 (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
Synonym(s):
Anti-CFIM, CFIM68, HPBRII-4, Anti-Cleavage and polyadenylation specificity factor 68 kDa, Anti-Pre-mRNA cleavage factor Im 68 kDa subunit, Anti-Protein HPBRII-4/7
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
~68 kDa
species reactivity
human
concentration
~1.0 mg/mL
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CPSF6(11052)
General description
The cleavage and polyadenylation specificity factor subunit 6 (CPSF6) protein is a subunit of the cleavage factor Im complex (CFIm). It contains an N-terminal RNP-type RNA binding domain (RBD) and the C-terminal region is rich in arginine-serine (RS), arginine- aspartic acid (RD) and arginine-glutamic acid (RE) repeats like pre-mRNA splicing serine-arginine (SR). CPSF6 is considered as a human immunodeficiency virus (HIV)-1 restriction factor. The CPSF6 gene is mapped to human chromosome 12q15.
Specificity
Anti-CPSF6 (N-terminal) recognizes human CPSF6.
Immunogen
synthetic peptide corresponding to the N-terminal region of human CPSF6
Application
Anti-CPSF6 (N-terminal) antibody produced in rabbit is suitable for:
- immunoblotting
- immunoprecipitation
- immunofluorescence
Biochem/physiol Actions
The cleavage and polyadenylation specificity factor subunit 6 (CPSF6) protein is involved in alternative polyadenylation, which is a part of the co-transcriptional processing of pre-mRNA in eukaryotes. The RNP-type RNA binding domain (RBD) is necessary for binding to the 25 kDa subunit. The C-terminal region of this protein mediates the interaction in vitro with shuttling serine-arginine (SR) proteins. CPSF6 is implicated in human immunodeficiency virus (HIV)-1 replication. The capsid (CA) protein of the virus requires CPSF6 for nuclear entry and integration site targeting.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Storage and Stability
Store at -20°C. For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Sabine Dettwiler et al.
The Journal of biological chemistry, 279(34), 35788-35797 (2004-06-01)
Cleavage factor I(m) (CF I(m)) is required for the first step in pre-mRNA 3'-end processing and can be reconstituted in vitro from its heterologously expressed 25- and 68-kDa subunits. The binding of CF I(m) to the pre-mRNA is one of
LRRFIP1, a new FGFR1 partner gene associated with 8p11 myeloproliferative syndrome.
G Soler et al.
Leukemia, 23(7), 1359-1361 (2009-04-17)
Michael Tellier et al.
BMC research notes, 12(1), 554-554 (2019-09-04)
Transcription of eukaryotic protein-coding genes by RNA polymerase II (pol II) is highly regulated at initiation, elongation and termination. Transcription is also coordinated with co-transcriptional processing of the emerging pre-mRNA by capping, splicing, and cleavage and polyadenylation. Polyadenylation (poly(A)) site
Akatsuki Saito et al.
Virology, 532, 118-126 (2019-05-10)
The HIV-1 capsid (CA) utilizes CPSF6 for nuclear entry and integration site targeting. Previous studies demonstrated that the HIV-1 CA C-terminal domain (CTD) contains a highly conserved K182 residue involved in interaction with CPSF6. In contrast, certain HIV-2 strains possess
David Alejandro Bejarano et al.
eLife, 8 (2019-01-24)
Nuclear entry of HIV-1 replication complexes through intact nuclear pore complexes is critical for successful infection. The host protein cleavage-and-polyadenylation-specificity-factor-6 (CPSF6) has been implicated in different stages of early HIV-1 replication. Applying quantitative microscopy of HIV-1 reverse-transcription and pre-integration-complexes (RTC/PIC)
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