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SAB4200258

Sigma-Aldrich

Anti-CPSF6 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-CFIM, CFIM68, Anti-HPBRII-4, Anti-Pre-mRNA cleavage factor Im 68 kDa subunit, Anti-Protein HPBRII-4/7, Anti-cleavage and polyadenylation specificity factor 68 kDa

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~68 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 2.5-5 μg using HEK-293T cell lysates
indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HeLa cells
western blot: 1-2 μg/mL using lysates of MCF7 cells

UniProt accession no.

Q16630

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CPSF6(11052)

General description

Anti-CPSF6 is produced in rabbit using as immunogen a synthetic peptide corresponding to an internal sequence in human CPSF6 conjugated to KLH. cleavage and polyadenylation specificity factor 6 (CPSF6) is a 68 kDa subunit. It contains an N-terminal ribonuclear particle (RNP)-type RNA binding domain (RBD), which is necessary for binding to the 25 kDa subunit. The C-terminal region of CPSF 6 is rich in Arg-Ser (RS), RD and RE repeats like pre-mRNA splicing serine and arginine-rich (SR) proteins.

Immunogen

synthetic peptide corresponding to an internal sequence in human CPSF6

Application

Anti-CPSF6 antibody produced in rabbit has been used:
  • immunoblotting
  • immunofluorescence
  • immunoprecipitation

Biochem/physiol Actions

The C-terminal region of cleavage and polyadenylation specificity factor 6 (CPSF 6) mediates the interaction in vitro with shuttling serine and arginine-rich (SR) proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
021M4769

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Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization
Dettwiler S, et al.
Test, 279(34), 35788-35797 (2004)
TNIP2 is a hub protein in the NF-kappaB network with both protein and RNA mediated interactions
Banks CAS, et al.
Molecular and Cellular Proteomics, 15(11), 3435-3449 (2016)
CPSF6 defines a conserved capsid interface that modulates HIV-1 replication
Price AJ, et al.
PLoS Pathogens, 8(8), e1002896-e1002896 (2012)
Charles A S Banks et al.
Molecular & cellular proteomics : MCP, 15(11), 3435-3449 (2016-11-03)
The NF-κB family of transcription factors is pivotal in controlling cellular responses to environmental stresses; abnormal nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling features in many autoimmune diseases and cancers. Several components of the NF-κB signaling pathway have

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