SAB4200254

Anti-SIRT6 antibody, Mouse monoclonal

clone SIRT6-4, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-NAD-dependent deacetylase sirtuin-6, Monoclonal Anti-SIR2-like protein 6, Monoclonal Anti-SIR2L6, Monoclonal Anti-sirtuin 6
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: SIRT6-4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~40 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: suitable; western blot: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human SIRT6.
• isotype: IgG2b
• UniProt accession no.: Q8N6T7
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SIRT6(51548) ; mouse ... Sirt6(50721)

Description

General description

Monoclonal Anti-SIRT6 (mouse IgG2b isotype) is derived from the hybridoma SIRT6-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminal region of human Sirt6 conjugated to KLH. Sirtuin 6 (Sirt6) belongs to the mammalian Sir2 gene family. It is a nuclear, chromatin-associated protein. Sirt6 is broadly expressed with the highest levels in muscle, brain and heart.

Immunogen

synthetic peptide corresponding to the N-terminal region of human Sirt6 (GeneID: 51548), conjugated to KLH.

Application

Monoclonal Anti-SIRT6 antibody produced in mouse has been used in immunoblotting.

Biochem/physiol Actions

Sirtuin 6 (Sirt6) plays a key role in DNA repair and maintenance of genomic stability in cells. Sirt6 is enzymatically inactive as a Nicotinamide adenine dinucleotide (NAD⁺) -dependent protein deacetylase but displays auto-adenosine triphosphate (ADP)-ribosyl transferase activity. Loss of Sirt6 leads to the development of an acute degenerative aging-like phenotype.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable