SAB4200246

Anti-Ambra1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-activating molecule in BECN1-regulated autophagy protein 1, Anti-autophagy/beclin 1 regulator 1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~130 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 2-4 μg/mL using HEK-293T cells overexpressing human Ambra1.
• UniProt accession no.: Q9C0C7
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... AMBRA1(55626) ; mouse ... Ambra1(228361) ; rat ... Ambra1(59319)

Description

General description

Activating molecule in Beclin 1-regulated autophagy (Ambra1) is a WD40-containing protein.[1] It is mainly expressed in the neuroepithelium during early neurogenesis. Ambra1 is highly conserved in vertebrates.[1] The gene is located on human chromosome 11p11.2.[2]

Immunogen

synthetic peptide corresponding to the N-terminal region of human Ambra1, conjugated to KLH. The corresponding sequence is identical in mouse and rat Ambra1.

Application

Anti-Ambra1 antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Activating molecule in Beclin 1-regulated autophagy (Ambra1) is a positive regulator of Beclin1-regulated autophagy. It is involved in the development of the nervous system. Upon autophagic stimuli, Ambra1 binds to Beclin1 and promotes the interaction of Beclin1 with its target phosphatidylinositol 3-kinase VPS34, thereby inducing autophagy. Functional deficiency of Ambra1 in mouse embryos is associated with severe neural tube defects such as autophagy impairment, unbalanced cell proliferation, accumulation of ubiquitinated proteins and excessive apoptosis.[1]

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable