SAB4200238

Anti-LAP2α antibody, Mouse monoclonal

clone 3A3, purified from hybridoma cell culture

• Synonym(s): Anti-LEM domain containing 4, Anti-LEMD4, Anti-Lamina-associated polypeptide 2, Anti-TMPO, Anti-TP α, Anti-Thymopoietin isoform α, Anti-Thymopoietin-related peptide isoform α
• MDL number: MFCD02263594
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 3A3, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~80 kDa
• species reactivity: human, bovine
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using whole extracts of human HeLa cells.
• UniProt accession no.: P42166
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TMPO(7112)

Description

General description

Monoclonal Anti-LAP2α (mouse IgG1 isotype) is derived from the hybridoma 3A3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide. Lamina-associated polypeptide 2, isoform α (LAP2α), also called thymopoietin isoform α, is encoded by the gene mapped to human chromosome 12q23.1. LAP2α is a LEM (LAP2-Emerin-MAN1) domain protein. that resides in the nucleus.

Specificity

Monoclonal Anti-LAP2α recognizes human and bovine LAP2α.

Immunogen

peptide corresponding to the C-terminus of human LAP2α, conjugated to KLH.

Application

Monoclonal Anti-LAP2α antibody produced in mouse may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Lamina-associated polypeptide 2 α (LAP2 α) may play a role in the assembly of the nuclear lamina, and thus helps in maintaining the structural organization of the nuclear envelope. It may also facilitate the binding of lamin A to the inner nuclear membrane. Mutation in this gene leads to the development of dilated cardiomyopathy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discardedif not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable