SAB4200229

Anti-CD3ζ chain antibody, Mouse monoclonal

clone TCR48, purified from hybridoma cell culture

• Synonym(s): Anti-CD247 antigen, Anti-CD3-eta, Anti-Cd3, Anti-Cd3h, Anti-T3z, Anti-TCRZ
• MDL number: MFCD03454876
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: TCR48, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~16 kDa
• species reactivity: human, mouse
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): suitable; western blot: 2.0-4.0 μg/mL using Jurkat or EL4 cell extracts
• isotype: IgG1
• UniProt accession no.: P20963
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Cd247(12503)

Description

General description

Monoclonal Anti- CD3ζ chain (mouse IgG1 isotype) is derived from the hybridoma TCR-48 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of mouse CD3ζ chain conjugated to KLH. CD3ζ is a 16-kDa transmembrane protein, which consists of a short extracellular domain and a long intracellular domain including immunoreceptor tyrosine-based activation motif (ITAM). It is a subunit of T cell receptor chains (TCR), which is expressed by T cells and natural killer (NK) cells.

Application

Monoclonal Anti-CD3ζ chain antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

CD3ζ is required for T cell receptor chains (TCR) complex assembly and expression. It is involved in immunity, neuronal development and synaptic plasticity. Downregulation of TCR ζ-chain expression and impairment of T-cell function is associated with systemic lupus erythematosus (SLE), rheumatoid arthritis, cancer and infectious diseases. Altered expression and function of CD3ζ in T cells is linked to hematological diseases.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable