SAB4200222

Anti-MAN1B1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-ER alpha 1,2-mannosidase, Anti-Man9GlcNAc2-specific processing alpha-mannosidase, Anti-alpha 1,2-mannosidase; endoplasmic reticulum alpha-mannosidase 1, Anti-endoplasmic reticulum alpha-mannosidase 1, Anti-endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, Anti-endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1, Anti-mannosidase, alpha, class 1B, member 1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~75 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human MAN1B1.
• UniProt accession no.: Q9UKM7
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAN1B1(11253)

Description

General description

Mannosidase α class 1B member 1 (MAN1B1), also known as endoplasmic reticulum α 1,2 mannosidase I (ERManI), is an ER resident transmembrane protein that belongs to the glycosyl hydrolase family 47. The MAN1B1 gene is mapped to human chromosome 9q34.3.

Specificity

Anti-MAN1B1 recognizes human MAN1B1.

Application

Anti-MAN1B1 antibody produced in rabbit is suitable for immunoblotting.

Biochem/physiol Actions

Mannosidase α class 1B member 1 (MAN1B1) gene is implicated in N-glycan processing and endoplasmic reticulum (ER) quality control. The MAN1B1 protein primarily removes the middle branch mannose from mannonanose-di-(N-acetyl-D-glucosamine) (Man₉GlcNAc₂) to form mannooctaose-di-(N-acetyl-D-glucosamine (Man₈GlcNAc₂) isomer B, but at high local concentrations, as found in the ER quality control compartment, it further trims the carbohydrates to Man_5-6GlcNAc₂. Overexpression of the MAN1B1 gene increases mannose trimming and accelerates endoplasmic reticulum associated degradation (ERAD) of terminally misfolded glycoproteins.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable