SAB4200219

Anti-CD3ζ chain antibody, Hamster monoclonal

clone H146-968, purified from hybridoma cell culture

• Synonym(s): Anti-CD247 antigen, Anti-CD3-eta, Anti-Cd3h, Anti-Cd3z, Anti-T3Z, Anti-TCRK, Anti-Tcrz, Monoclonal Anti-CD3ζ chain antibody produced in hamster
• MDL number: MFCD03454876
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: hamster
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: H146-968, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~16 kDa
• species reactivity: mouse, human
• concentration: ~1.0 mg/mL
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 2.5-5.0 μg/mL using Jurkat or EL4 cell extracts
• isotype: IgG2
• UniProt accession no.: P20963
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Cd247(12503)

Description

General description

Monoclonal Anti-CD3 ζ chain (hamster IgG2 isotype) is derived from the hybridoma H146-968 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from an immunized American hamster. The T-cell surface glycoprotein CD3 ζ (CD3 ζ) chain is a subunit of the T-cell receptor (TCR) heterodimer. It consists of immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domains.

Specificity

Monoclonal Anti-CD3 ζ chain recognizes human and mouse CD3 ζ chain.

Application

Monoclonal Anti-CD3ζ chain antibody produced in hamster has been used in western blotting.
It is also suitable for:

• immunoprecipitation
• immunocytochemistry
• flow cytometry

Biochem/physiol Actions

The T-cell receptor (TCR) is responsible for antigen recognition and signal transduction, which are significantly part of the antigen-specific immune responses. TCR integrity is crucial for stimulating optimal and efficient immune responses, along with pathogen elimination and the elimination of modified cells and molecules. The ζ-chain subunit of the TCR mediates the receptor assembly, expression and signaling. Impaired TCR ζ-chain expression and T-cell function is known to be associated with various chronic pathologies, including cancer, infectious diseases and autoimmune disorders.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable