SAB4200214
Anti-Deptor antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
Synonym(s):
Anti-DEP domain containing 6, Anti-DEP domain containing mTOR interacting protein, Anti-DEPDC6
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~48 kDa
species reactivity
mouse, human, canine, rat
concentration
~1.0 mg/mL
technique(s)
western blot: 0.5-1.0 μg/mL using whole extracts of human ACHN and mouse A9 cells
western blot: 1-2 μg/mL using whole extracts of rat RAT2 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DEPTOR(64798)
mouse ... Deptor(97998)
General description
DEPTOR (DEP domain containing MTOR-interacting protein) is a tumor suppressor and mTOR-binding protein. This protein is expressed in vascular endothelial cells.
Deptor is encoded by the gene mapped to human chromosome 8q24.12.
Deptor is made up of disheveled/egl-10/pleckstrin (DEP) domains and one post-synaptic density-95/discs large/zonula occludens-1 (PDZ) domain.
Specificity
Anti-Deptor recognizes human, mouse, rat, and canine Deptor.
Application
Anti-Deptor antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
DEPTOR (DEP domain containing MTOR-interacting protein) suppresses mTORC1 (mammalian target of rapamycin complex 1) and mTORC2 proteins which are responsible for the control of cell growth, metabolism, proliferation and survival. It is expressed in normal pancreatic acinar and islet cells, and this expression is completely lost in pancreatic ductal adenocarcinoma (PDAC) tissues. Therefore, it functions as a tumor suppressor in pancreatic cancer. It is regulator of inflammatory and angiogenic responses and controls the activation of vascular endothelial cells. DEPTOR is expressed in placenta, and DEPTOR signaling is influenced by maternal stress which might eventually impact the outcome of pregnancy. The expression of this protein is reduced in thyroid, prostate, bladder and cervical cancer.
Overexpression of the DEPTOR gene inhibits mTORC1. The feedback inhibition of phosphatidylinositol-3-kinase (PI3K), leading to the activation of the PI3K/mTORC2/protein kinase B (Akt) pathway. This protein is highly expressed in multiple myelomas fostering cyclin D1/D3 or c-musculoaponeurotic fibrosarcoma (c-MAF)/musculoaponeurotic fibrosarcoma (MAFB) translocations.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Storage and Stability
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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High expression levels of the mammalian target of rapamycin inhibitor DEPTOR are predictive of response to thalidomide in myeloma.
Boyd K D, et al.
Leukemia & Lymphoma, 51(11), 2126-2129 (2010)
Timothy R Peterson et al.
Cell, 137(5), 873-886 (2009-05-19)
The mTORC1 and mTORC2 pathways regulate cell growth, proliferation, and survival. We identify DEPTOR as an mTOR-interacting protein whose expression is negatively regulated by mTORC1 and mTORC2. Loss of DEPTOR activates S6K1, Akt, and SGK1, promotes cell growth and survival
DEPTOR regulates vascular endothelial cell activation and proinflammatory and angiogenic responses.
Bruneau S et al
Blood, 122(10), 1833-1842 (2013)
Placental DEPTOR as a stress sensor during pregnancy.
Mparmpakas D et al
Clinical Science (London, England : 1979), 122(7), 349-359 (2012)
Marina P Volegova et al.
PloS one, 18(9), e0292080-e0292080 (2023-09-28)
Improper regulation of translation initiation, a vital checkpoint of protein synthesis in the cell, has been linked to a number of cancers. Overexpression of protein subunits of eukaryotic translation initiation factor 3 (eIF3) is associated with increased translation of mRNAs
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