SAB4200208

Anti-RNF5 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-DAAP-218M18.2, Anti-E3 ubiquitin-protein ligase RNF5, Anti-Protein G16, Anti-RING5, Anti-RMA1, Anti-Ring finger protein 5, Anti-hsRma1, Anti-ram1 homolog
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~20 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 2.5-5.0 μg/mL using whole extracts of human HeLa cells
• UniProt accession no.: Q99942
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RNF5(6048) ; mouse ... Rnf5(54197) ; rat ... Rnf5(407784)

Description

General description

Ring-finger protein 5 (RNF5), also known as RMA1, is a membrane-bound RING finger E3 ubiquitin ligase, which is conserved from worm to human. This protein is restricted to the dense bodies and the M-line of the body wall muscles. RNF5 is composed of a classic really interesting new gene (RING) domain, a single transmembrane-spanning domain and a formin-like homology domain. The RNF5 gene is mapped to human chromosome 6p21.32.

Specificity

Anti-RNF5 recognizes human RNF5.

Application

Anti-RNF5 antibody produced in rabbit is suitable for immunoblotting.

Biochem/physiol Actions

Ring-finger protein 5 (RNF5) is a component of the endoplasmic reticulum associated degradation (ERAD) machinery, where it has a role in ubiquitin-dependent degradation of misfolded proteins, such as mutant cystic fibrosis transmembrane conductance regulator (CFTR), as a part of the cell protein quality control. Deregulation of the RNF5 gene in endoplasmic reticulum (ER) stress is associated with muscular disorders. RNF5 regulates cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. RNF5 is also involved in the regulation of cellular antiviral responses. High expression of the RNF5 gene is detected in breast cancer tumors and in melanoma, leukemia, ovarian and renal tumor-derived cell lines.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable