SAB4200205

Anti-Tensin 3 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-TEM6, Anti-TNS3, Anti-Tensin-like SH2 domain-containing protein 1, Anti-Tumor endothelial marker 6
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~180 kDa
• species reactivity: human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1.5-3.0 μg/mL using HEK-293T cell lysates over expressing human tensin 3.
• UniProt accession no.: Q68CZ2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TNS3(64759)

Description

General description

Tensin 3 is expressed in various tissues such as thyroid, kidney and placenta and different cell types including endothelial, epithelial cells and fibroblasts.

Tensin-3 gene (TNS3) is located on human chromosome 7p12.3. TNS3 has N-terminal actin-binding domain and C-terminal sarcoma (Src) homology 2 (SH2) domain and a phosphotyrosine binding (PTB) domain.

Specificity

Anti-Tensin3 (C-terminal) specifically recognizes human tensin 3.

Application

Anti-Tensin 3 (C-terminal) antibody produced in rabbit may be used in immunoblotting.[1]

Biochem/physiol Actions

Tensin 3 contributes to cell migration and tumorigenesis in cell lines from advanced lung cancer, breast cancer and melanoma.

Tensin-3 (TNS3) plays a key role in cell adhesion and migration by crosslinking actin to adhesion proteins like integrin. Phosphorylation of tensin-3 by sarcoma (Src) tyrosine kinase is observed in carcinomas. Downregulation of tensin-3 is implicated in the metastatic behavior of human kidney carcinoma. Knockdown of TNS3 gene impairs adhesion and migration of breast cancer cells.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable