SAB4200199

Anti-DFF45 antibody ,Mouse monoclonal

clone 3F12, purified from hybridoma cell culture

• Synonym(s): Anti-DFFA, Anti-DNA fragmentation factor, 45kDa, alpha polypeptide, Anti-H13, DFF1, Anti-ICAD
• MDL number: MFCD02096208
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 3F12, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~37 kDa; antigen ~45 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using whole extracts of human G361 cells
• isotype: IgG1
• UniProt accession no.: O00273
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DFFA(1676)

Description

General description

Monoclonal Anti-DFF45 (mouse IgG1 isotype) is derived from the hybridoma 3F12 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.
The gene DNA fragmentation factor subunit α (DFFA) is mapped to human chromosome 1p36.2. The encoded protein localizes to the chromatin fraction. It exists in association with DFF40, thereby forming a heterodimer. DFFA is popularly called as DFF45 (DNA fragmentation factor 45).

Specificity

Monoclonal Anti-DFF45 recognizes human DFF45 isoform 1 and isoform 2.

Immunogen

human DFF45 recombinant protein

application

Anti-DFF45 antibody, Mouse monoclonal is suitable for immunoblotting.

Biochem/physiol Actions

DNA fragmentation factor subunit alpha (DFFA) is a component of DNA fragmentation factor (DFF). It is mainly associated with apoptosis and genomic stability. Caspase-3-mediated cleavage of DFFA releases DFF40 (DNA fragmentation factor 40) which degrades chromosomal DNA. During menstruation, the apoptosis of endometrium cells is associated with DFFA. Its expression decreases after menopause. Silencing of DFFA increases doxorubicin effects in breast cancer cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at -20 oC. For continuous use,the product may be stored at 2-8oCfor up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discardedif not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable