Monoclonal Anti-Importin 4 (rat IgG2a isotype) is derived from the hybridoma 3C2 produced by the fusion of mouse myeloma cells and splenocytes from immunized rat.
Specificity
Monoclonal Anti-importin 4 recognizes human, mouse, rat, monkey and hamster importin 4.
Immunogen
mouse importin 4 fusion protein.
Application
Monoclonal Anti-Importin 4 antibody produced in rat is suitable for immunoblotting and immunocytochemistry.
Biochem/physiol Actions
Importin-4 is involved in the nuclear import of ribosomal protein S3a (rpS3a), as well as vitamin D receptor (VDR). Furthermore, importin-4 has been found to mediate the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical nuclear localization signal (NLS). Importin-4 is also involved in transporting transition protein 2 (TP2) and interacts with the NLS sequence of TP2 in the spermatid. A three-dimensional (3D) structure of importin-4 indicates that the three helices (helices 21 to 23) of the importin-4 protein contribute to the NLS of TP2 binding pocket.
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at -20 °C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage freeze at -20 °Cinworking aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Molecular and cellular biology, 28(13), 4331-4341 (2007-08-08)
Mammalian spermiogenesis is characterized by a unique chromatin-remodeling process in which histones are replaced by transition protein 1 (TP1), TP2, and TP4, which are further replaced by protamines. We showed previously that the import of TP2 into the haploid spermatid
Many nuclear transport pathways are mediated by importin beta-related transport receptors. Here, we identify human importin (Imp) 4b as well as mouse Imp4a, Imp9a and Imp9b as novel family members. Imp4a mediates import of the ribosomal protein (rp) S3a, while
The Journal of biological chemistry, 280(49), 40901-40908 (2005-10-07)
Vitamin D receptor (VDR) is localized in nuclei and acts as a ligand-dependent transcription factor. To clarify the molecular mechanisms underlying the nuclear translocation of VDR, we utilized an in vitro nuclear transport assay using digitonin-permeabilized semi-intact cells. In this
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