Monoclonal Anti-CACYBP (mouse IgG1 isotype) is derived from the hybridoma CABP7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein. The protein is a human ortholog of mouse calcyclin (S100A6)-binding protein and an element of novel ubiquitin ligase complex.
The gene CACYBP (calcyclin binding protein) is mapped to human chromosome 1q25.1. It is widely expressed in human tissues.
Specificity
Monoclonal Anti-CACYBP recognizes human, dog, bovine, and monkey CACYBP.
Application
Anti-CACYBP antibody, Mouse monoclonal is suitable for immunoblotting.
Biochem/physiol Actions
CACYBP (calcyclin binding protein) is a part of the ubiquitination machinery and is involved in the degradation of β-catenin. It can interact with S100, SIAH1 (seven in absentia homolog 1) and SKP1 (S-phase kinase-associated protein 1). CACYBP is involved in proliferation, differentiation, multidrug resistance and tumorigenicity. It can work as an oncogene and as a tumor suppressor. In glioma cells, CACYBP enhances the cellular proliferation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Storage and Stability
Store at −20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The Journal of biological chemistry, 277(32), 28848-28852 (2002-06-04)
Recently, a human ortholog of mouse calcyclin (S100A6)-binding protein (CacyBP) called SIP (Siah-1-interacting protein) was shown to be a component of a novel ubiquitinylation pathway regulating beta-catenin degradation (Matsuzawa, S., and Reed, J. C. (2001) Mol. Cell 7, 915-926). In
Genetic aberrations in prostate cancer by microarray analysis.
Saramaki OR, et al.
International Journal of Cancer. Journal International Du Cancer, 119, 1322-1329 (2006)
Molecular cancer research : MCR, 5(12), 1254-1262 (2008-01-04)
Calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP), a target protein of the S100 family, which includes S100A6, S100A1, S100A12, S100B, and S100P, has been identified as a component of a novel ubiquitinylation complex leading to beta-catenin degradation. However, the function of CacyBP/SIP in
Expression and regulation of CacyBP/SIP in chronic lymphocytic leukemia cell balances of cell proliferation with apoptosis.
Fu C, et al.
Journal of Cancer Research and Clinical Oncology, 142, 741-748 (2016)
CacyBP/SIP protein is important for the proliferation of human glioma cells.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
