SAB4200184
Anti-WTX (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
Synonym(s):
Anti-AMER1, Anti-FAM123B, Anti-FLJ39827, Anti-OSCS, Anti-Wilms tumor gene on the X chromosome
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200 μL
$413.32
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200 μL
$413.32
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~190 kDa
species reactivity
human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
western blot: 1-2 μg/mL using HEK-293T cell lysates over expressing human WTX.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FAM123B(139285)
General description
APC membrane recruitment protein 1 (AMER1) or Wilms Tumor on the X (WTX) gene, also known as FAM123B, comprises adenomatous polyposis coli (APC) interacting domains, nuclear localization signal (NLS), phospholipid binding region, six conserved block sequences and β-catenin binding region. The N-terminal region harbors the internal splicing sites (donor and acceptor). It encodes three isoforms AMER1, AMER2 and AMER3 APC, by alternative splicing at the N-terminus. WTX gene is localized on the X chromosome. The phosphatidylinositol (4,5)-bisphosphate binding site of the WTX aids its localization to the plasma membrane.
Specificity
Anti-WTX (N-terminal) specifically recognizes human WTX.
Application
Anti-WTX (N-terminal) antibody produced in rabbit has been used in chromatin immunoprecipitation[1] and immunoblotting.
Biochem/physiol Actions
APC membrane recruitment protein 1 (AMER1) or WTX is localized in the subnuclear compartment and participates in RNA processing, transcription and cell differentiation. In cultured cells, WTX negatively regulates the WNT/β-catenin signaling by promoting β-catenin ubiquitination and thus targets its degradation by the proteasome. WTX has been shown to form a complex with β-catenin, Axis inhibition protein 1 (AXIN1), β-transducin repeat containing protein 2 (β-TrCP2) and adenomatous polyposis coli (APC). WTX mutations are majorly somatic and the gene deletions and truncations is implicated in 30% of Wilms tumors. The mutations were confined to the active X and single X chromosomes in females and males respectively.. Loss of WTX function in Wilms tumor results in stabilization of β-catenin and its translocation to the nucleus. WTX co-localizes with non-POU domain-containing octamer-binding protein (p54nrb/NONO) and binds Wilms′ tumor protein 1 (WT1) to modulate its activity, suggesting that WTX may play a role in the transcriptional regulation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Agnès Boutet et al.
BMC evolutionary biology, 10, 280-280 (2010-09-17)
WTX is a novel gene mutated in a proportion of Wilms' tumors and in patients suffering from sclerosing bone dysplasia. On the molecular level WTX has been shown to act as an antagonist of canonical Wnt/β-catenin signaling in fish and
Zandra A Jenkins et al.
Nature genetics, 41(1), 95-100 (2008-12-17)
Abnormalities in WNT signaling are implicated in a broad range of developmental anomalies and also in tumorigenesis. Here we demonstrate that germline mutations in WTX (FAM123B), a gene that encodes a repressor of canonical WNT signaling, cause an X-linked sclerosing
Miguel N Rivera et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(20), 8338-8343 (2009-05-07)
WTX encodes a tumor suppressor gene inactivated in Wilms tumor and recently implicated in WNT signaling through enhancement of cytoplasmic beta-catenin (CTNNB1) degradation. Here, we report that WTX translocates to the nucleus, a property that is modified by an endogenous
Thomas L Clarke et al.
Molecular cell, 65(5), 900-916 (2017-02-28)
Protein post-translation modification plays an important role in regulating DNA repair; however, the role of arginine methylation in this process is poorly understood. Here we identify the arginine methyltransferase PRMT5 as a key regulator of homologous recombination (HR)-mediated double-strand break
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