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SAB4200170

Anti-CPSF4 antibody produced in rabbit

Name: Anti-CPSF4 antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR 4, Anti-CPSF30NS1 EFFECTOR DOMAIN-BINDING PROTEIN 1, Anti-NEB1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~30 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

western blot: 1-2 μg/mL using Whole extracts of HEK-293T cells overexpressing human CPSF4.

UniProt accession no.

O95639

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CPSF4(10898)
mouse ... CPSF4(54188)
rat ... CPSF4(304277)

General description

Cleavage polyadenylation specificity factor 4 (CPSF4), also called CPSF 30 is a component of the CPSF complex. It comprises of five CCCH zinc finger motifs (ZF1-ZF5). CPSF4 gene is mapped to human chromosome 7q22.1.

Specificity

Anti-CPSF4 recognizes human CPSF4.

Application

Anti-CPSF4 antibody produced in rabbit has been used in chromatin immunoprecipitation and immunoblotting.

Biochem/physiol Actions

Cleavage polyadenylation specificity factor 4 (CPSF4) is a component of 3′-end mRNA processing machinery and binds to poly(U) sequence of RNA via the zinc finger motifs. These motifs are also involved in protein-protein interactions. CPSF4 along with other proteins primarily coordinate both cleavage and polyadenylation. Their interaction with the non-structural protein 1 (NS1) protein of the influenza virus leads to inhibition of polyadenylation event and 3′ end cleavage of pre-mRNA associated with the host. Elevated expression of CPSF4 is observed in lung adenocarcinomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Upregulation of cleavage and polyadenylation specific factor 4 in lung adenocarcinoma and its critical role for cancer cell survival and proliferation

Chen W, et al.

PLoS ONE, 8(12), e82728-e82728 (2013)

Identifying the molecular signature of the interstitial deletion 7q subgroup of uterine leiomyomata using a paired analysis.

Jennelle C Hodge et al.

Genes, chromosomes & cancer, 48(10), 865-885 (2009-07-16)

Uterine leiomyomata (UL), the most common neoplasm in reproductive-age women, have recurrent cytogenetic abnormalities including interstitial deletion of 7q. To develop a molecular signature, matched del(7q) and non-del(7q) tumors identified by FISH or karyotyping from 11 women were profiled with

3? end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells

Davidson L, et al.

Genes & Development, 28(4), 342-356 (2014)

3' end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells.

Lee Davidson et al.

Genes & development, 28(4), 342-356 (2014-01-31)

3' end formation of pre-mRNAs is coupled to their transcription via the C-terminal domain (CTD) of RNA polymerase II (Pol II). Nearly all protein-coding transcripts are matured by cleavage and polyadenylation (CPA), which is frequently misregulated in disease. Understanding how

Protein factors in pre-mRNA 3'-end processing.

C R Mandel et al.

Cellular and molecular life sciences : CMLS, 65(7-8), 1099-1122 (2007-12-26)

Most eukaryotic mRNA precursors (premRNAs) must undergo extensive processing, including cleavage and polyadenylation at the 3'-end. Processing at the 3'-end is controlled by sequence elements in the pre-mRNA (cis elements) as well as protein factors. Despite the seeming biochemical simplicity

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