The amyotrophic lateral sclerosis 2 (ALS2) C-terminal like (ALS2CL) gene is an ALS2 homologous gene, encoding a homo-dimeric protein. The ALS2CL protein forms an ALS2-ALS2CL heteromeric complex with ALS2 oligomer. This complex has been shown to co-localize in membrane compartments. The ALS2CL is mapped to human chromosome 3p21.31.
Specificity
Anti-ALS2CL specifically recognizes human ALS2CL.
Application
Anti-ALS2CL antibody produced in rabbit is suitable for immunoblotting.
Biochem/physiol Actions
The amyotrophic lateral sclerosis 2 (ALS2) C-terminal like (ALS2CL) protein exhibits a weak Ras-related protein Rab-5A (Rab5)-guanine nucleotide exchange factor (GEF) activity, but strongly binds to Rab5. The co-expression of ALS2CL and Rab5 causes the distinctive tubulation phenotype of endosome compartments. It also results in the co-localization of ALS2CL and Rab5. Also, the ALS2CL protein suppresses the endosome enlargement induced by ALS2, resulting in an extensive perinuclear tubulo-membrane phenotype. In contrast to ALS2, ALS2CL might be considered as a critical moiety in modulating the ALS2 and Rab5-mediated endosome dynamics in the cell.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freezeat -20°C, in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
ALS2, the causative gene product for juvenile recessive amyotrophic lateral sclerosis (ALS2), is a guanine-nucleotide exchange factor for the small GTPase Rab5. Here, we report a novel ALS2 homologous gene, ALS2 C-terminal like (ALS2CL), which encodes a 108-kD ALS2CL protein.
Biochemical and biophysical research communications, 354(2), 491-497 (2007-01-24)
ALS2, the causative gene product for a number of recessive motor neuron diseases, is a guanine-nucleotide exchange factor for Rab5, and acts as a modulator for endosome dynamics. Recently, we have identified a novel ALS2 homolog, ALS2CL, which is highly
Arteriosclerosis, thrombosis, and vascular biology, 39(5), 902-914 (2019-03-29)
Objective- The topographical distribution of atherosclerosis in vasculature underscores the importance of shear stress in regulating endothelium. With a systems approach integrating sequencing data, the current study aims to explore the link between shear stress-regulated master transcription factor and its
Questions
Reviews
★★★★★ No rating value
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
