SAB4200163

Anti-WTX (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-AMER1, Anti-FAM123B, Anti-OSCS, Anti-Wilms tumor gene on the X chromosome
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~190 kDa (additional ~150 kDa band may be observed corresponding to alternatively spliced WTX form)
• species reactivity: human
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): western blot: 1-2 μg/mL using using cell lysates of HEK-293T overexpressing human WTX.
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FAM123B(139285)

Description

General description

The Wilms tumor gene on the X chromosome (WTX) gene encodes two protein forms resulting from alternative splicing at the N-terminus. The encoded protein localizes between the cytoplasm and nucleus and is present in a distinct subnuclear compartment. The WTX gene is mapped to human chromosome Xq11.2.

Specificity

Anti-WTX (C-terminal) specifically recognizes human WTX.

Application

Anti-WTX (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

The Wilms tumor gene on the X chromosome (WTX) gene is found targeted by deletions and truncations in up to 30% of Wilms tumor. Wilms tumor is the most common pediatric kidney cancer and a pluripotent tumor that arises from a kidney-specific stem cell population. All known WTX mutations are somatic, targeting the single X chromosome in males or the active X chromosome in females, thus leading to its complete inactivation. WTX has been shown to form a complex with β-catenin, axis inhibition protein 1 (AXIN1), β-transducin repeat containing protein 2 (β-TrCP2), and anaphase-promoting complex (APC). In cultured cells, WTX negatively regulates the wingless-related integration site (Wnt) /β-catenin signaling by promoting β-catenin ubiquitination and thus targets its degradation by the proteasome. Loss of WTX function in Wilms tumor results in the stabilization of β-catenin and its translocation to the nucleus. WTX colocalizes with p54nrb/ non-POU domain-containing octamer-binding protein (NonO) and binds WT1 to modulate its activity, suggesting that WTX may play a role in the transcriptional regulation of cell differentiation. It is also implicated RNA processing.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at -20 °C.For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable