SAB4200158

Monoclonal Anti- MAGE-11

clone 4E7, tissue culture supernatant, isotype IgG2a

• Synonym(s): Monoclonal Anti-Mage-11 antibody produced in mouse, Melanoma antigen family A, 11, CT1.11, MAGEA-11, MAGEA11
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• conjugate: unconjugated
• antibody form: tissue culture supernatant
• antibody product type: primary antibodies
• clone: 4E7, monoclonal
• form: buffered aqueous solution
• mol wt: ~47 kDa
• species reactivity: rat, human, sheep, bovine, monkey, mouse, hamster, canine
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 1:250-1:500 using HeLa cell extracts; western blot: suitable
• isotype: IgG2a
• UniProt accession no.: P43364
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAGEA11(4110) ; mouse ... 4930550L24Rik(75352) ; rat ... Magea13(302845)

Description

General description

Monoclonal Anti-MAGE-11 (rat IgG2a isotype) is derived from the hybridoma 4E7 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a fusion protein corresponding to a fragment of human melanoma associated gene-11 (MAGE-11). In human, the MAGE family of genes includes about 25 genes. The MAGE-A family consists of 12 proteins. This gene is mapped o human chromosome Xq28.

Specificity

Monoclonal Anti-MAGE-11 recognizes human, monkey, bovine, mouse, rat, hamster, sheep, and dog MAGE-11.

Application

Monoclonal Anti- MAGE-11 has been used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry

Biochem/physiol Actions

Melanoma associated gene-11 (MAGE-A) is identified as one of the cancer-testis antigens in some embryonic tissues. MAGE-A acts as a coregulator of androgen receptor (AR) expression in human female reproductive tract. It is involved in neuronal development and apoptosis. MAGE-A11 has been found mainly in the nucleus of cancer cells. Interestingly, MAGE-11 interferes with tumor angiogenesis and glycolytic switch by affecting the hypoxia-inducible factors (HIFs) activation.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative, containing 10% fetal calf serum.

Storage and Stability

Store at –20 °C. For continuous use, the product maybe stored at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable