SAB4200157
Anti-TIN2 antibody, Mouse monoclonal
clone MT-28, purified from hybridoma cell culture
Synonym(s):
Anti-TERF1 (TRF1)-interacting nuclear factor 2, Anti-TIN2L, AntiTINF2, Monoclonal Anti-TIN2 antibody produced in mouse
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
MT-28, monoclonal
form
buffered aqueous solution
mol wt
antigen ~40 kDa
species reactivity
rat, mouse
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
western blot: 0.5-1 μg/mL using using L929 cell extracts
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Tinf2(28113)
rat ... Tinf2(290232)
General description
Monoclonal Anti-TIN2 (mouse IgG2b isotype) is derived from the hybridoma MT-28 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Telomeric repeat-binding factor TRF)-interacting nuclear factor 2 (TIN2) is a component of the shelterin or telosome complex. The TIN2 gene is mapped to human chromosome 14q12. It exists as two isoforms TIN2L and TIN2S.
Specificity
Monoclonal Anti-TIN2 recognizes mouse and rat TIN2.
Immunogen
synthetic peptide corresponding to amino acids 216-330 of mouse TIN-2, conjugated to KLH. The corresponding sequence differs by two amino acids in rat.
Application
Monoclonal Anti-TIN2 antibody produced in mouse may be used in immunoblotting.
Biochem/physiol Actions
Telomeric repeat-binding factor TRF1-interacting nuclear factor 2 (TIN2) interacts with the TRF homology (TRFH) domain of telomeric repeat factor 1 (TRF1) and tethers tripeptidyl peptidase 1 (TPP1)/ protection of telomeres 1 (POT1) to TRF1 and TRF2. TIN2 also connects TRF1 to TRF2 and this link contributes to the stabilization of TRF2 on telomeres. In addition, TIN2 has also been found to tether the telomeres to the nuclear matrix. Shelterin/telosome, a six-subunit protein complex, which TIN2 is part of, protects chromosome ends from DNA damage response and regulates telomere length maintenance by telomerase. Interestingly, TIN2 has also been found to be mutated in Dyskeratosis Congenita, a multisystem disorder, characterized by abnormalities of the skin, nails and mucous membranes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at -20 °C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Takeshi Mizuno et al.
Genes to cells : devoted to molecular & cellular mechanisms, 26(6), 360-380 (2021-03-13)
Mouse telomerase and the DNA polymerase alpha-primase complex elongate the leading and lagging strands of telomeres, respectively. To elucidate the molecular mechanism of lagging strand synthesis, we investigated the interaction between DNA polymerase alpha and two paralogs of the mouse
M Soledad Fernández García et al.
Journal of blood medicine, 5, 157-167 (2014-08-30)
Dyskeratosis congenita (DC) is an inherited bone marrow failure (BMF) syndrome characterized by the classic triad of abnormal skin pigmentation, nail dystrophy, and oral leukoplakia. However, patients usually develop BMF and are predisposed to cancer, with increased risk for squamous
Jeffrey Zheng-Sheng Ye et al.
The Journal of biological chemistry, 279(45), 47264-47271 (2004-08-19)
Human telomeres contain two related telomeric DNA-binding proteins, TRF1 and TRF2. The TRF1 complex contains the TRF1 interacting partner, TIN2, as well as PIP1 and POT1 and regulates telomere-length homeostasis. The TRF2 complex is primarily involved in telomere protection and
Chiou Mee Kong et al.
The FEBS journal, 280(14), 3180-3193 (2013-05-08)
The discovery of telomeres dates back to the early 20th century. In humans, telomeres are heterochromatic structures with tandem DNA repeats of 5'-TTAGGG-3' at the chromosomal ends. Telomere length varies greatly among species and ranges from 10 to 15 kb
Patrick G Kaminker et al.
Cell cycle (Georgetown, Tex.), 8(6), 931-939 (2009-02-21)
Telomeres are specialized heterochromatin at the ends of linear chromosomes. Telomeres are crucial for maintaining genome stability and play important roles in cellular senescence and tumor biology. Six core proteins-TRF1, TRF2, TIN2, POT1, TPP1 and Rap1 (termed the telosome or
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