SAB4200144

Anti-AMSH/STAMBP antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Associated molecule with the SH3 domain of STAM, Anti-Endosome-associated ubiquitin isopeptidase, Anti-STAM-binding protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: human, rat, mouse
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 10-20 μg/mL using human HeLa cells; western blot: 2.5-5.0 μg/mL using whole extracts of mouse NIH-3T3 and rat NRK cells
• UniProt accession no.: O95630
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... STAMBP(10617) ; mouse ... Stambp(70527) ; rat ... Stambp(171565)

Description

General description

Associated molecule with the SH3 domain of STAM (AMSH), also known as signal transducing adapter molecule 1 (STAM) binding protein (STAMBP), belongs to the JAMM domain metalloprotease family of Zn²⁺ dependent deubiquitinating enzymes (DUBs). AMSH is composed of multiple protein-protein interaction domains. It also includes a nuclear localization signal (NLS), a microtubule-interacting and transport (MIT) domain, a clathrin-binding domain and a STAM interacting motif.

application

Anti-AMSH/STAMBP antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Associated molecule with the SH3 domain of STAM (AMSH) is involved in the deubiquitination of endosomal cargo preceding lysosomal degradation. AMSH specifically cleaves K-63-linked polyubiquitin chains and is implicated in the endocytic sorting of several receptors including G protein-coupled receptors and receptor tyrosine kinases. AMSH is also associated with the downregulation of epidermal growth factor. It interacts directly with clathrin. The interaction with clathrin is essential for AMSH endosomal localization.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable