SAB4200140
Anti-VILIP-1 (N-terminal) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody
Synonym(s):
Anti-HLP3, Anti-HPCAL3, Anti-HUVISL1, Anti-Hippocalcin-like protein3, Anti-VILIP, Anti-VSNL1, Anti-Visinin-like protein-1 (VLP-1)
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.44
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~22 kDa
species reactivity
mouse, rat, human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.5 mg/mL
technique(s)
western blot: 1.5-3.0 μg/mL using rat brain extracts (S1 fraction), mouse brain extracts (S1 fraction) and HEK-293T cell lysates overexpressing human VILIP-1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VSNL1(7447)
mouse ... Vsnl1(26950)
rat ... Vsnl1(24877)
General description
Visinin-like protein-1 (VILIP-1 also known as VSNL1, VILIP, HPCAL3, HUVISL1, HLP3), belongs to a large family of neuronal calcium (Ca2+) sensor (NCS) proteins. The VILIP subfamily includes VILIP-1, VILIP-2, VILIP-3, hippocalcin and neurocalcin-δ. VILIP-1 is primarily expressed in the brain, particularly in the hippocampus. The VILIP1 gene is mapped to human chromosome 2p24.2.
Specificity
Anti-VILIP-1 (N-terminal) specifically recognizes human, rat and mouse VILIP-1.
Application
Anti-VILIP-1 (N-terminal) antibody produced in rabbit is suitable for immunoblotting.
Biochem/physiol Actions
Neuronal Ca2+ sensor (NCS) proteins possess four EF-hand Ca2+-binding motifs that facilitate their association with lipid bilayers. They are involved in a variety of Ca2+-dependent signal transduction processes in neurons. Visinin-like protein-1 (VILIP-1) associates with the plasma membrane and Golgi membranes and modulates the signaling of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in neuronal cells. VILIP-1 upregulates the expression of functional α4β2 neuronal nicotinic acetylcholine receptors in hippocampal neurons. It controls the activation of guanylyl cyclase B through clathrin-dependent receptor cycling, supporting membrane trafficking in the central nervous system (CNS). VILIP-1 is associated with the pathology of CNS disorders including Alzheimer′s disease and schizophrenia. It also acts as a biomarker in ischemic stroke. VILIP-1 gene expression is found to be regulated during induction of hippocampal synaptic plasticity, underlying learning and memory processes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at −20 °C. For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers.is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Marian Brackmann et al.
Journal of cell science, 118(Pt 11), 2495-2505 (2005-06-01)
The family of neuronal Ca2+ sensor (NCS) proteins is known to influence a variety of physiological and pathological processes by affecting signalling of different receptors and ion channels. Recently, it has been shown that the NCS protein VILIP-1 influences the
C Zhao et al.
Neuroscience, 153(4), 1202-1212 (2008-04-29)
Visinin-like protein-1 (VILIP-1) belongs to the neuronal calcium sensor (NCS) family of EF-hand Ca(2+)-binding proteins which are involved in a variety of Ca(2+)-dependent signal transduction processes in neurons. VILIP-1 has been implicated in the pathology of CNS disorders including Alzheimer's
Lin Lin et al.
The Journal of biological chemistry, 277(44), 41872-41878 (2002-08-31)
The calcium sensor protein visinin-like protein-1 (VILIP-1) was isolated from a brain cDNA yeast two-hybrid library using the large cytoplasmic domain of the alpha4 subunit as a bait. VILIP-1 is a myristoylated calcium sensor protein that contains three functional calcium
Jacqueline Brown et al.
BMC cancer, 20(1), 281-281 (2020-04-08)
Esophageal squamous cell carcinoma (ESCC) is an aggressive cancer with one of the highest world incidences in the Eastern Cape region of South Africa. Several genome wide studies have been performed on ESCC cohorts from Asian countries, North America, Malawi
James B Ames et al.
Biochimica et biophysica acta, 1820(8), 1205-1213 (2011-10-25)
Neuronal calcium sensor (NCS) proteins, a sub-branch of the calmodulin superfamily, are expressed in the brain and retina where they transduce calcium signals and are genetically linked to degenerative diseases. The amino acid sequences of NCS proteins are highly conserved
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