SAB4200138

Anti-Synemin (C-terminal region) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-DMN, Anti-Desmuslin, Anti-SYN
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~170 kDa
• species reactivity: rat
• packaging: antibody small pack of 25 μL
• concentration: ~1.5 mg/mL
• technique(s): western blot: 1.5-3.0 μg/mL using using rat bladder extract (S1 fraction).
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Synm(308709)

Description

General description

Synemin exists in two isoforms, α and β.

Synemin is a 230kDa intermediate filament protein (IFp). It is expressed along with desmin and vimentin filaments in muscle cells. In mouse, synemin is encoded by the gene mapped in B5 region of chromosome 7. Alternative splicing of this gene produces three different isoforms with different molecular mass namely, high (180kDa), medium (150kDa) and low (41kDa). High and medium isoforms are expressed in embryonic mouse, whereas, low molecular weight protein is expressed in adult mouse. Synemin comprises ∼310-amino acid rod domain characteristic of IF proteins and 1,290 amino acid tail domain at its C- terminal.

Specificity

Anti-Synemin (C-terminal region) specifically recognizes rat synemin.

Application

Anti-Synemin (C-terminal region) antibody produced in rabbit is suitable for immunoblotting.

Biochem/physiol Actions

α-Synemin is essential for the stabilization of junctional complexes at sarcolemma, in neonates. β-Synemin controls the connection of desmin with Z-disks. Synemin is also involved in protein phosphorylation, for regulating key signaling cascades in the heart. Mild myopathy has been observed in synemin knockout mice.

Synemin in association with either desmin or vimentin plays a vital role in cytoskeletal organization and also facilitates function of intermediate filaments in erythrocytes and muscle. Interaction of synemin and vinculin aids in attachment of intermediate filaments (Ifs) to the cell membrane.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at -20 °C.For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation.Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable