SAB4200126

Anti-MALT1 antibody ,Mouse monoclonal

clone CLone 31E, purified from hybridoma cell culture

• Synonym(s): Anti-MLT, Anti-MLT1, Anti-mucosa associated lymphoid tissue lymphoma translocation gene
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: CLone 31E, monoclonal
• form: buffered aqueous solution
• mol wt: ~90 kDa
• species reactivity: rat, monkey, human
• concentration: ~1.5 mg/mL
• technique(s): western blot (chemiluminescent): 2-4 μg/mL using A549, COS7 and 293T total cell extracts
• isotype: IgG2b
• UniProt accession no.: Q9UDY8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MALT1(10892) ; rat ... Malt1(307366)

Description

General description

Monoclonal Anti-MALT1 (mouse IgG2b isotype) is derived from the hybridoma 31E produced by the fusion of mouse myeloma cells and splenocytes from the immunized BALB/c mice. The gene for mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) is located on human chromosome 18q21 and encodes for a human paracaspase. It is characterized by a death domain in its amino terminus, two immunoglobulin (Ig)-like domains, and a p20-caspase-like domain in its carboxyl terminus.

Specificity

Monoclonal Anti-MALT1 recognizes human, monkey and rat MALT1

Immunogen

fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human MALT1

Application

Anti-MALT1 antibody ,Mouse monoclonal is suitable for immunoblotting and immunohistochemistry.

Biochem/physiol Actions

The mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) acts as an important regulator of adaptive immune responses in vivo and is essential for functional T-cell activation, proliferation and cytokine production. The activation of MALT1 is physiologically mediated by B cell lymphoma 10 (BCL10), which forms a complex with MALT1, promoting the oligomerization and activation of the caspase-like domain. In vivo and in vitro studies suggest that the MALT1-BCL10 complex mediates nuclear factor κB (NF-κB) activation in response to surface antigen receptor activation in both B and T cells. The most common is the translocation t(11;18)(q21;q21) which results in a fusion of the cellular inhibitor of apoptosis protein 2 (cIAP2) region on chromosome 11q21 with the MALT1 gene and is present in more than one-third of cases.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discardedif not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable