SAB4200121

Anti-Annexin 4 antibody,Mouse monoclonal

clone ANX47, purified from hybridoma cell culture

• Synonym(s): Anti-ANX4, Anti-ANXA4, Anti-PIG28, Anti-Placental anticoagulant protein II, Anti-ZAP36, Anti-Zymogen granule membrane-associated protein, 36-KD
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: ANX47, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 34 kDa
• species reactivity: mouse, bovine, human, monkey
• packaging: antibody small pack of 25 μL
• concentration: ~1.5 mg/mL
• technique(s): immunohistochemistry: suitable; western blot: 2-4 μg/mL using A431 total cell extracts
• isotype: IgG1
• UniProt accession no.: P09525
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ANXA4(307)

Description

General description

Annexin A4 is encoded by the gene mapped to human chromosome 2p13.3. The encoded protein belongs to the Ca²⁺-regulated and phospholipid-binding annexin superfamily proteins. Annexin A4 is highly expressed in epithelial cells.

Monoclonal Anti-Annexin 4 (mouse IgG1 isotype) is derived from the hybridoma ANX47 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human Annexin 4 protein.

Immunogen

recombinant human Annexin 4 protein

Application

The product may be used in several immunochemical techniques including immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Elevated expression of annexin A4 leads to development of various cancers such as colorectal cancer (CRC), esophageal squamous cell carcinoma (ESCC), endometrial carcinoma (EC), gastric cancer (GC), chemoresistant lung cancer (LC), malignant mesothelioma (MM), renal cell carcinoma (RCC), ovarian clear cell carcinoma (OCCC), cholangiocarcinoma, hepatocellular carcinoma (HCC), breast cancer (BC) and laryngeal cancer. Thus, annexin A4 can be used in diagnosis, prognosis and treatment of these cancers. In addition, the encoded protein acts as a tumor suppressor for prostate cancer (PCa) and has a contradictory role for pancreatic cancer (PCC).

The major involvement of Annexin 4 is seen in membrane permeability, exocytosis and regulation of ion channels. Its role in membrane fluidity and membrane trafficking may explain in part the involvement of Annexin 4 in modulating drug resistance in cancer cells. Indeed, Annexin 4 was found to confer chemoresistance in ovarian cancer cells in part by enhancing drug efflux.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable