SAB4200111

Anti-TARBP2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-TRBP, Anti-TRBP1, Anti-Tar (HIV-1) RNA binding protein 2
• MDL number: MFCD08705573
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~30 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HeLa cells; western blot: 2-4 μg/mL using lysates of HEK-293T cells
• UniProt accession no.: Q15633
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TARBP2(6895) ; mouse ... 21357(21357) ; rat ... Tarbp2(363006)

Description

General description

The transactivating response (TAR) RNA-binding protein (TRBP) (also known as TARBP2) gene is mapped to human chromosome 12q13.13. The encoded protein exists in two isoforms, TRBP1 and TRBP2, where TRBP2 has 21 additional amino acids. It is composed of two double stranded RNA binding domains (dsRBDs), a KR-helix motif within dsRBD2 and a Medipal domain that binds Moesin-ezrin-radixin-like protein (Merlin), Dicer and protein activator of the interferon-induced protein kinase (PACT). TRBP is a component of the RNA-induced silencing complex (RISC) loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), composed of Dicer and argonaute (Ago).

Specificity

Anti-TRBP2 recognizes human TRBP2.

Immunogen

synthetic peptide corresponding to amino acids 183-195 of human TRBP2 conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Application

Anti-TARBP2 has been used in immunoblotting and might also be used in immunofluorescence.

Biochem/physiol Actions

The transactivating response (TAR) RNA-binding protein (TRBP) (also known as TARBP2) binds and activates the human immunodeficiency virus (HIV)-1 with TAR RNA. It plays a role in spermatogenesis, cell growth, oncogenesis and viral replication. Targeted disruption of the TARBP2 gene, causes growth defects, sterility problems and severe oligospermic effects in mice.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids