SAB4200110

Anti-PIWIL1 (internal region) antibody produced in rabbit

~1.0 mg/mL, buffered aqueous solution

• Synonym(s): Anti-HIWI, Anti-MIWI, Anti-PIWI-like 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~95 kDa
• species reactivity: human, rat, mouse
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: suitable using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections and biotin/ExtrAvidin^®-Peroxidase staining system; western blot: 1-2 μg/mL using rat and mouse testis extracts
• UniProt accession no.: Q96J94
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PIWIL1(9271) ; mouse ... Piwil1(57749) ; rat ... Piwil1(363912)

Description

General description

The PIWIL1 (Piwi like RNA-mediated gene silencing 1) gene encodes an 861 amino acid protein, which belongs to the Piwi subfamily of Argonaute proteins. This gene is mapped to human chromosome 12q24.33. The Piwi proteins are mostly localized to the germline, where they bind to PIWI-interacting RNAs (piRNAs). The Piwi proteins contain the Piwi-Argonaute-Zwille (PAZ) domain and the PIWI domain.

Specificity

Anti-PIWIL1 (internal region) recognizes human, mouse, and rat PIWIL1.

Application

Anti-PIWIL1 (internal region) antibody produced in rabbit is suitable for immunoblotting and immunohistochemistry.

Biochem/physiol Actions

The Piwi proteins are important for the biogenesis and function of small non-coding RNAs. These proteins and PIWI-interacting RNAs (piRNAs) have been implicated in epigenetic control of gene expression, transposon silencing, gene expression, and translation regulation. The Piwi proteins significantly participate in germline development and gametogenesis of various metazoan species. These proteins undergo symmetrical dimethyl arginines (sDMAs) modification by protein methyltransferase 5 (PRMT5).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilutions should be discarded if not used within 12 hours.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable